ArticleLineage-DeterminingTranscriptionFactorTCF-1InitiatestheEpigeneticIdentityofTCellsGraphicalAbstractHighlightsdTCF-1drivestheearlywaveofchromatinaccessibilityinTcelldevelopmentdTcf7�/�micecannotestablishtheopenchromatinlandscapeofnormalTcellsdAtthesingle-celllevel,TCF-1dictatesacoordinateopeningofthechromatindTCF-1caneraserepressivemarksandactivateTcell-restrictedgenesinfibroblastsAuthorsJohnL.Johnson,GeorgiosGeorgakilas,JelenaPetrovic,...,AvinashBhandoola,E.JohnWherry,GolnazVahediCorrespondencevahedi@pennmedicine.upenn.eduInBriefItisknownthatTCF-1isrequiredforTcelldevelopment,butthemechanismbywhichitcontrolstheTcelllineageremainsunclear.Johnsonetal.revealthatTCF-1controlsTcellfatethroughitsabilitytocreatedenovoopenchromatin,establishingtheepigeneticidentityofTcells.Johnsonetal.,2018,Immunity48,243–257February20,2018ª2018ElsevierInc.https://doi.org/10.1016/j.immuni.2018.01.012ImmunityArticleLineage-DeterminingTranscriptionFactorTCF-1InitiatestheEpigeneticIdentityofTCellsJohnL.Johnson,1,2,3,7GeorgiosGeorgakilas,1,2,3,7JelenaPetrovic,2,4,7MakotoKurachi,2,5StanleyCai,1,2,3ChristelleHarly,6WarrenS.Pear,2,4AvinashBhandoola,6E.JohnWherry,2,5andGolnazVahedi1,2,3,8,*1DepartmentofGenetics2InstituteforImmunology3EpigeneticsInstitute4DepartmentofPathologyandLaboratoryMedicine5DepartmentofMicrobiologyPerelmanSchoolofMedicine,UniversityofPennsylvania,Philadelphia,PA19104,USA6NationalCancerInstitute,NationalInstitutesofHealth,Bethesda,MD20184,USA7Theseauthorscontributedequally8Leadauthor*Correspondence:vahedi@pennmedicine.upenn.eduhttps://doi.org/10.1016/j.immuni.2018.01.012SUMMARYTcelldevelopmentisorchestratedbytranscriptionfactorsthatregulatetheexpressionofgenesinitiallyburiedwithininaccessiblechromatin,butthetran-scriptionfactorsthatestablishtheregulatoryland-scapeoftheTcelllineageremainunknown.ProfilingchromatinaccessibilityateightstagesofTcelldevel-opmentrevealedtheselectiveenrichmentofTCF-1atgenomicregionsthatbecameaccessibleattheearlieststagesofdevelopment.TCF-...
