Seeonlineversionforlegendandreferences.SnapShot:MacromolecularMachinesMichelaG.Bertero,ErikVerschueren,BernhardPaetzold,andLuisSerrranoCenterforGenomicRegulation,UniversitatPompeuFabra,08003Barcelona,Spain652Cell143,November12,2010©2010ElsevierInc.DOI10.1016/j.cell.2010.10.035123456789101112131415103514NUCLEUSNUCLEOLUSCYTOSOLNUCLEUSCYTOPLASMPDB|EMDB3RNApolymeraseII1Y1W4RNApolymeraseI14356RNApolymeraseIII13227Tau60/DTau912J041226SProteasome1RYP11Hsp104proteinchaperone13581Nucleosome2ID315F0F1ATPsynthase2WPD13ESCRT-Icore2P229Ribosome3JYW5Microtubulenucleator,�:TuSCrings17312ClampandDNAslidingclamp1SXJ14CytochromeBC11KB98Exportin-1/tRNA/RanGTP3ICQ10exosome2WP8SPINDLEPOLEBODYSPINDLEPOLEBODYNUCLEOLUSNUCLEOLUSENDOPLASMICRETICULUMENDOPLASMICRETICULUMMITOCHONDRIAMITOCHONDRIAGOLGIGOLGIVACUOLEVACUOLE13245671112141589101011121313652.e1Cell143,November12,2010©2010ElsevierInc.DOI10.1016/j.cell.2010.10.035SnapShot:MacromolecularMachinesMichelaG.Bertero,ErikVerschueren,BernhardPaetzold,andLuisSerrranoCenterforGenomicRegulation,UniversitatPompeuFabra,08003Barcelona,SpainProteinsrarelyworkalone.Instead,toperformaparticularcellulartask,theytypicallyinteracttoformfunctionalassembliescalled“complexes.”Genome-widestudiesonprotein-proteininteractionshaverevealedaround800proteincomplexesinthemodelorganismSaccharomycescerevisiae(vonMeringetal.,2002;Gavinetal.,2002,2006).Thechallengenowistounderstandthestructureandfunctionofthesemacromolecularmachines.Structuralbiologymethods,includingnuclearmagneticresonance(NMR),X-raycrystallography,electronmicroscopy,andtomography,catchsnapshotsofthesemolecularmachinesinactionandthusprovidethethree-dimensionalorganizationofthesecomplexesatonemomentintime.In2005,theEuropeanCommission6thFrameworkPro-grammefundedaconsortiumcalled3DRepertoire(http://www.3drepertoire.org),whosegoalistosolvestructuresofallproteincomplexesfromS.cerevisiae.Todate,thecon-sortiumhasfurnished~50structuresofproteincomplexes.Bycombiningthesestructureswiththosesolvedbyotherlaboratories,wecannowstartbuildingathree-dimensionalreconstructionofthewholecell.ThisSnapShotdisplays15ofthemostrelevantmacromolecularcellularmachinesintheyeastcell.RefeRencesGavin,A.C.,Bösche,M.,Krause,R.,Grandi,P.,Marzioch,M.,Bauer,A.,Schultz,J.,Rick,J.M.,Michon,A.M.,Cruciat,C.M.,etal.(2002).Functionalorganizationoftheyeastpro-teomebysystematicanalysisofproteincomplexes.Nature415,141–147.Gavin,A.C.,Aloy,P.,Grandi,P.,Krause,R.,Bösche,M.,Marzioch,M.,Rau,C.,Jensen,L.J.,Bastuck,S.,Dümpelfeld,B.,etal.(2006).Proteomesurveyrevealsmodularityoftheyeastcellmachinery.Nature440,631–636.vonMering,C.,Krause,R.,Snel,B.,Cornell,M.,Oliver,S.G.,andBork,P.(2002).Comparativeassessmentoflarge-scaledatasetsofprotein-proteininteractions.Nature417,399–403.
