2Cell???,??MONTH????DATE??,200?©200?ElsevierInc.DOIXXXXXXXXXSeeonlineversionfor???SnapShot:XXXXXXXXXXXXXXXXAUTHORXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAFFILIATIONXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXWBCPlateletsMitoticCTCApoptoticCTCEndothelialcellsEpithelialCTCMesenchymalCTCCTMVMCTCsDisseminationmechanismsPotentialclinicalutility*Typically1CTCper1-10millionwhitebloodcells(WBCs)*RedbloodcellsnotshownforclarityCirculatingtumormicroemboli(CTM)Adhesionsignalingandenhancedsurvival?Cellcomposition:homotypicandheterotypicImmunecellinteractions?EpithelialtomesenchymaltransitionVasculogenicmimicryCollectivemigrationCellco-operationSingle-cellmolecularprofilingProteins:Phenotypicmarkers,therapytargetsDNA:Tumor-specifcgenomes,copynumberaberrations,whole-exomesequencing,whole-genomesequencingRNA:Pathwayactivation,therapytargetsTargetdiscoveryPrognosticbiomarkerPredictivebiomarkerPatientmonitoringDrugresponses/screeningCTCenrichmentNoenrichmentSurface-marker-basedcapture,e.g.,EpCamMarkerindependentbasedoncellphysicalcharacteristics,e.g.,size,deformabilityAnalyzeallcellsCTCisolationDirectmanualpickingoruseofsemi-automateddevicestoassistinobtainingcellsviacellcontactormanipulationofcellchargeHowCTCsarestudied25µmMesenchymalCTCsDapiECs/WBCsCTCsinNSClungcancer742Cell168,February9,2017©2017ElsevierInc.DOIhttp://dx.doi.org/10.1016/j.cell.2017.01.026SeeonlineversionforlegendsandreferencesSnapShot:CirculatingTumorCellsCarolineDiveandGedBradyCancerResearchUKManchesterInstitute,UniversityofManchester,ManchesterM204BX,UK742.e1Cell168,February9,2017©2017ElsevierInc.DOIhttp://dx.doi.org/10.1016/j.cell.2017.01.026SnapShot:CirculatingTumorCellsCarolineDiveandGedBradyCancerResearchUKManchesterInstitute,UniversityofManchester,ManchesterM204BX,UKCTCstoStudyTumorBiology:ARenaissanceDeliveredbyTechnologyAdvancesThediscoveryofcirculatingtumorcells(CTCs)bytheAustralianpathologistJohnAshworthin1869tooksometimetogaintraction,largelybecauseofthesignificanttechnicalchallengeof...
