Seeonlineversionforlegendandreferences.PartIIwillappearinanewEnhancedSnapShotformatintheOctober2issue.1248Cell138,September18,2009©2009ElsevierInc.DOI10.1016/j.cell.2009.08.001SnapShot:AntibioticInhibitionofProteinSynthesisIDanielSohmen,1JoergM.Harms,2FrankSchlünzen,3andDanielN.Wilson1,41UniversityofMunich,Germany;2MPSD,UniversityofHamburg,Germany;3DESY,Hamburg,Germany;4CiPS-M,Munich,GermanyClassNameTargetMechanismSmall(30S)RibosomalSubunitAminoglycosidesApramycin,gentamycin,hygromycinB,kanamycin,neomycin,paromomycin,tobramycinElongation(translocation)Mostaminoglycosideantibioticsinducetranslationalmisreadingbypromotingbindingofnear-cognatetRNAs,butthebiologicaleffectisprobablyduetoinhibitionofthetranslocationreactionandpromotionofback-translocation.EdeineEdeineAInitiation(fMet-tRNAbinding)EdeinepreventsbindingoftheinitiatortRNAtothe30SsubunitinanmRNA-dependentmanner.GE81112GE81112Initiation(fMet-tRNAbinding)GE81112inhibitsbindingoftheinitiatortRNAtothe30SsubunitinanmRNA-independentmanner.KasugamycinKasugamycinInitiation(fMet-tRNAbinding)KasugamycininhibitstranslationinitiationofcanonicalmRNAsbybindinginthepathofthemRNAandpreventingstableinteractionoftheinitiatortRNAwiththestartcodon.PactamycinPactamycinInitiation,elongation(translocation)Earlydatasuggestthatpactamycinallows30Sbutnot70Sinitiationcomplexformation,whereasrecentlypactamycinwasshowntoinhibittranslocationinatRNA-mRNA-dependentmanner.SpectinomycinSpectinomycinElongation(translocation)Spectinomycinbindstotheneckofthesmallsubunitandpreventstherelativemovementoftheheadandbodythatisneededforthecompletionoftranslocation.StreptomycinStreptomycinElongation(misreading)StreptomycinincreasesaffinityoftRNAfortheAsite,hasamodestinhibitoryeffectontranslocation,promotesback-translocation,andinduceshigh-leveltranslationalmisreading.Tetracyclines/glycylcyclinesDoxycycline,minocycline,tetracycline/tigecyclineElongation(tRNAdelivery)TetracyclinespreventstablebindingoftheEF-Tu-tRNA-GTPternarycomplextotheribosomeandinhi...
