ArticleAssemblyandFunctionofHeterotypicUbiquitinChainsinCell-CycleandProteinQualityControlGraphicalAbstractHighlightsdK11/K48-bispecificantibodiesdetectendogenousK11/K48-linkedubiquitinchainsdK11/K48-branchedchainsmodifymitoticregulatorsandmisfoldednascentproteinsdK11/K48-dependentproteasomaldegradationpreventsproteinaggregationdMutationsinK11/K48-specificenzymesarefoundinneurodegenerativediseasesAuthorsRichardG.Yau,KerstinDoerner,ErickR.Castellanos,...,MarissaL.Matsumoto,VishvaM.Dixit,MichaelRapeCorrespondencematsumoto.marissa@gene.com(M.L.M.),dixit.vishva@gene.com(V.M.D.),mrape@berkeley.edu(M.R.)InBriefBispecificantibodiesrevealthepresenceandfunctionofheterotypicubiquitinchainscontainingK11andK48linkagesincell-cycleregulationandproteinqualitycontrol.Yauetal.,2017,Cell171,918–933November2,2017ª2017ElsevierInc.https://doi.org/10.1016/j.cell.2017.09.040ArticleAssemblyandFunctionofHeterotypicUbiquitinChainsinCell-CycleandProteinQualityControlRichardG.Yau,1,2KerstinDoerner,1ErickR.Castellanos,3DianeL.Haakonsen,1,2AchimWerner,1NanWang,6,7X.WilliamYang,6,7NadiaMartinez-Martin,5MarissaL.Matsumoto,3,*VishvaM.Dixit,4,*andMichaelRape1,2,8,*1DepartmentofMolecularandCellBiology,UniversityofCaliforniaatBerkeley,Berkeley,CA,USA2HowardHughesMedicalInstitute,Berkeley,CA,USA3DepartmentofStructuralBiology,GenentechInc.,SouthSanFrancisco,CA,USA4DepartmentofPhysiologicalChemistry,GenentechInc.,SouthSanFrancisco,CA,USA5DepartmentofMicrochemistry,Proteomics,andLipidomics,GenentechInc.,SouthSanFrancisco,CA,USA6CenterforNeurobehavioralGenetics,SemelInstituteforNeuroscience&HumanBehavior,UniversityofCalifornia,LosAngeles,LosAngeles,CA,USA7DepartmentofPsychiatryandBiobehavioralSciences,DavidGeffenSchoolofMedicineatUCLA,UniversityofCalifornia,LosAngeles,LosAngeles,CA,USA8LeadContact*Correspondence:matsumoto.marissa@gene.com(M.L.M.),dixit.vishva@gene.com(V.M.D.),mrape@berkeley.edu(M.R.)https://doi.org/10.1016/j.cell.2017.09.040SUMMARYPosttranslationalmodificationwithubiquitinchainscontro...
