Nlrp9binflammasomerestrictsrotavirusinfectioninintestinalepithelialcellsShuZhu1,*,SiyuanDing2,3,*,PenghuaWang4,ZhengWei1,WenPan5,NoahWPalm1,YiYang1,HuaYu1,Hua-BingLi1,GengWang1,XuqiuLei1,MarcelR.deZoete1,JunZhao1,6,YunjiangZheng1,HaiweiChen1,YujiaoZhao4,KellieA.Jurado7,NingguoFeng2,LiangShan1,YuvalKluger6,JunLu5,ClaraAbraham7,ErolFikrig7,8,HarryB.Greenberg2,3,andRichardA.Flavell1,81DepartmentofImmunobiology,YaleUniversitySchoolofMedicine,NewHaven,Connecticut06520,USA2DepartmentofMedicineandDepartmentofMicrobiology&Immunology,StanfordUniversitySchoolofMedicine,Stanford,California94305,USA3PaloAltoVeteransInstituteofResearch,VAPaloAltoHealthCareSystem,PaloAlto,California94304,USA4DepartmentofMicrobiologyandImmunology,NewYorkMedicalCollege,Valhalla,NewYork10595,USA5DepartmentofGenetics,YaleUniversitySchoolofMedicine,NewHaven,Connecticut06520,USA6DepartmentofPathology,YaleUniversitySchoolofMedicine,NewHaven,Connecticut06520,USA7DepartmentofInternalMedicine,YaleUniversitySchoolofMedicine,NewHaven,Connecticut06520,USA8HowardHughesMedicalInstitute,ChevyChase,Maryland20815,USAReprintsandpermissionsinformationisavailableatwww.nature.com/reprints.CorrespondenceandrequestsformaterialsshouldbeaddressedtoR.A.F.(richard.flavell@yale.edu).*Theseauthorscontributedequallytothiswork.OnlineContent:Methods,alongwithanyadditionalExtendedDatadisplayitemsandSourceData,areavailableintheonlineversionofthepaper;referencesuniquetothesesectionsappearonlyintheonlinepaper.SupplementaryInformationisavailableintheonlineversionofthepaper.AuthorContributions:S.Z.wrotethemanuscript,designed,performedandinterpretedexperimentswithtechnicalassistancefromS.D.andP.H.W;S.D.andH.B.G.providedrotavirusrelatedreagents,S.D.performedtherotavirusfecalsheddingassay,qPCRandimmunostainingexperiments;P.H.W,W.PandY.ZperformedRNAbindingexperiments;Z.W.andH.C.performedinfectionsinintestinalorganoids;Y.Y.andH.YperformedTUNELstaining;H.-B.L.andK.A.J.helpedwithDHX9functionalstudies;G.W.,X.L.andY.Z.assistedinthecharacter...
