Genomiccopynumberdictatesagene-independentcellresponsetoCRISPR-Cas9targetingAndrewJ.Aguirre1,2,3,4*,RobinM.Meyers2*,BarbaraA.Weir1,2,FranciscaVazquez1,2,Cheng-ZhongZhang1,2,UriBen-David2,AprilCook1,2,GavinHa1,2,WilliamF.Harrington2,MihirB.Doshi1,2,MariaKost-Alimova2,StanleyGill1,2,HanXu2,LeviD.Ali2,GuozhiJiang2,SashaPantel2,YenaraeLee2,AmyGoodale2,AndrewD.Cherniack2,CoyinOh2,GregoryKryukov1,2,GlennS.Cowley2,LeviA.Garraway1,2,3,4,5,KimberlyStegmaier1,2,4,6,CharlesW.Roberts2,7,ToddR.Golub1,2,4,5,MatthewMeyerson1,2,4,8,9,DavidE.Root2,AviadTsherniak2#,WilliamC.Hahn1,2,3,4,9#1Dana-FarberCancerInstitute,450BrooklineAvenue,Boston,MA02215USA2BroadInstituteofHarvardandMIT,415MainStreet,Cambridge,MA02142USA3DepartmentofMedicine,BrighamandWomen’sHospitalandHarvardMedicalSchool,75FrancisStreet,Boston,MA021154HarvardMedicalSchool,25ShattuckStreet,Boston,Massachusetts02115,USA.5HowardHughesMedicalInstitute,4000JonesBridgeRoad,ChevyChase,Maryland20815,USA.6BostonChildren’sHospital,Boston,MA,USA7St.JudeChildren’sResearchHospital,262DannyThomasPlace,Memphis,TN8DepartmentofPathology,HarvardMedicalSchool,Boston,Massachusetts,USA.9CenterforCancerGenomeDiscovery,Dana-FarberCancerInstitute,Boston,MA,USA*Theseauthorscontributedequally#Co-correspondingauthorsWilliamC.Hahn,M.D.,Ph.D.,450BrooklineAvenue,Dana1538,Boston,MA02215USA,617-632-2641(phone),617-632-4005(fax),william_hahn@dfci.harvard.eduAviadTsherniak,BroadInstituteofHarvardandMIT,415MainStreet,Cambridge,MA02142USA,617-714-7506(phone),aviad@broadinstitute.orgRunningTitle:GenomiccopynumberimpactsCRISPR-Cas9screensResearch.onJune6,2016.©2016AmericanAssociationforCancercancerdiscovery.aacrjournals.orgDownloadedfromAuthormanuscriptshavebeenpeerreviewedandacceptedforpublicationbuthavenotyetbeenedited.AuthorManuscriptPublishedOnlineFirstonJune3,2016;DOI:10.1158/2159-8290.CD-16-01542ConflictofInterestDisclosure:LeviA.GarrawayandWilliamC.HahnareconsultantsforNovartis.MatthewMeyersonreceivesresearchsupportfromBayer.Research.onJune6,20...
