DOI:10.1126/science.1246981,80(2014);343Scienceetal.TimWangGeneticScreensinHumanCellsUsingtheCRISPR-Cas9SystemThiscopyisforyourpersonal,non-commercialuseonly.clickinghere.colleagues,clients,orcustomersby,youcanorderhigh-qualitycopiesforyourIfyouwishtodistributethisarticletoothershere.followingtheguidelinescanbeobtainedbyPermissiontorepublishorrepurposearticlesorportionsofarticles):January3,2014www.sciencemag.org(thisinformationiscurrentasofThefollowingresourcesrelatedtothisarticleareavailableonlineathttp://www.sciencemag.org/content/343/6166/80.full.htmlversionofthisarticleat:includinghigh-resolutionfigures,canbefoundintheonlineUpdatedinformationandservices,http://www.sciencemag.org/content/suppl/2013/12/11/science.1246981.DC1.htmlcanbefoundat:SupportingOnlineMaterialhttp://www.sciencemag.org/content/343/6166/80.full.html#relatedfoundat:canberelatedtothisarticleAlistofselectedadditionalarticlesontheScienceWebsiteshttp://www.sciencemag.org/content/343/6166/80.full.html#ref-list-1,19ofwhichcanbeaccessedfree:cites43articlesThisarticleregisteredtrademarkofAAAS.isaScience2014bytheAmericanAssociationfortheAdvancementofScience;allrightsreserved.ThetitleCopyrightAmericanAssociationfortheAdvancementofScience,1200NewYorkAvenueNW,Washington,DC20005.(printISSN0036-8075;onlineISSN1095-9203)ispublishedweekly,exceptthelastweekinDecember,bytheScienceonJanuary3,2014www.sciencemag.orgDownloadedfromonJanuary3,2014www.sciencemag.orgDownloadedfromonJanuary3,2014www.sciencemag.orgDownloadedfromonJanuary3,2014www.sciencemag.orgDownloadedfromonJanuary3,2014www.sciencemag.orgDownloadedfromonJanuary3,2014www.sciencemag.orgDownloadedfrom(RCA),whichisnegativelyregulatedbyproteinphosphatase1(PP1)(25).WepostulatethatRCAentailstheinteractionoftheH4tailwithneigh-boringnucleosomesandthatPP1counteractsthisprocessbydephosphorylatingH3S10,aknownsubstrateofthisenzyme(26).Wethereforepro-posethat,togetherwithcondensin,thesignalingcascadeweidentifiedisaconservedmechanismforshapingmitoticchromosomes.Ref...
