实验方法总结（2）：细胞生物学部分1.原代细胞分离与培养....................................................................................21.1原代细胞分离................................................................................................21.2原代细胞培养................................................................................................22.干细胞分离培养..............................................................................................32.1胚胎干细胞诱导分化.....................................................................................32.2肿瘤干细胞（CSC）分离鉴定......................................................................33.细胞增殖相关研究方法...................................................................................53.1MTT分析法...................................................................................................53.2XTT................................................................................................................53.3WST-1...........................................................................................................63.4CCK-8(WST-8).............................................................................................63.4平板克隆形成...............................................................................................63.5软琼脂克隆形成............................................................................................73.6BrdU.............................................................................................................74.细胞转移相关研究方法................................................................................84.1划痕..............................................................................................................84.2细胞粘附.......................................................................................................84.3Transwell迁移.....................................................................
