《食品工业》2023年第44卷第2期291分析检测标准加入法与标准曲线法测定松茸中总砷含量的比较黄智安*，沈蕊，罗欣，余进，田浩，陈彦和劲牌有限公司，中药保健食品质量与安全湖北省重点实验室（黄石435100）摘要对比标准加入法和标准曲线法,采用电感耦合等离子体质谱仪(ICP-MS)测定松茸中总砷含量。结果显示,标准曲线法和标准加入法的线性关系均良好,相关系数R2均在0.9990以上,检出限分别为0.053ng/mL和0.037ng/mL,定量限分别为0.176ng/mL和0.123ng/mL。以称取0.5g样品,定容至50mL计算,采用标准曲线法和标准加入法测定砷的检出限分别为0.0053mg/kg和0.0037mg/kg。2种方法测定松茸中总砷含量,测定结果无明显差异,相对标准偏差SRSD＜5%,并使用有证标准物质紫菜GBW10023进行验证,测定结果均在标准值范围内。同时,对测定过程的干扰因素进行验证,松茸中较低含量的Sm、Nd所产生的双电荷离子150Sm++、150Nd++对砷的测定无干扰。因此,采用电感耦合等离子体质谱仪测定松茸中总砷的方法准确、可靠。标准加入法作为标准曲线法的补充,可有效分析这类复杂基体样品测定的准确性。关键词电感耦合等离子体质谱仪;标准加入法;标准曲线法;松茸;总砷ComparisonofDeterminationofTotalArsenicinTricholomamatsutakebystandardAdditionMethodandStandardCurveMethodHUANGZhian*,SHENRui,LUOXin,YUJin,TIANHao,CHENYanheJingBrandCo.,Ltd.,HubeiProvincialKeyLabforQualityandSafetyofTraditionalChineseMedicineHealthFood(Huangshi435100)AbstractComparingthestandardadditionmethodwiththestandardcurvemethod,thecontentoftotalarsenicinTricholomamatsutakewasdeterminedbyinductivelycoupledplasmamassspectrometry(ICP-MS).Theresultsshowedthatthelinearityofstandardcurvemethodandstandardadditionmethodwasgood,thecorrelationcoefficientR2wasabove0.9990,thedetectionlimitswere0.053ng/mLand0.037ng/mLrespectively,andthequantitativelimitswere0.176ng/mLand0.123ng/mL,respectively.Byweighing0.5gsampleandmeasuringfixedcapacityto50mL,thedetectionlimitofarsenicwas0.0053mg/kgand0.0037mg/kgbystandardcurvemethodandstandardadditionmethod,respectively.TherewasnosignificantdifferencebetweenthetwomethodsforthedeterminationoftotalarsenicinTricholomamatsutake,andtherelativestandarddeviationwaslessthan5%.Thecertifiedreferencemateria...
