福建畜牧兽医第45卷第4期2023年鸡毒支原体活疫苗渊F36株冤的免疫效果评估张惠云兆丰华生物科技渊福州冤有限公司福州350014摘要鸡毒支原体在国内鸡场的感染率较高袁疫苗免疫是防控鸡毒支原体的有效方法之一遥本试验旨在观察鸡毒支原体活疫苗渊F36株冤免疫鸡群的安全性和有效性遥选择体重相近的7日龄SPF鸡80羽袁免疫组点眼免疫鸡毒支原体活疫苗渊F36株冤袁对照组点眼免疫生理盐水袁于38日龄对免疫组和对照组鸡群采用鸡毒支原体R株攻毒袁攻毒后每天观察鸡群采食尧精神状况尧呼吸道症状和死亡情况袁分别于38日龄尧52日龄对两组鸡进行称重袁7日龄尧14日龄尧21日龄尧38日龄尧52日龄采血检测抗体袁52日龄采集咽喉拭子做PCR检测袁并于52日龄将所有鸡剖杀袁做气囊病理性损伤和增重检查遥结果表明院鸡毒支原体活疫苗渊F36株冤免疫鸡群后袁未见明显免疫副反应与呼吸道症状袁免疫组相对于对照组增重显著渊P约0.05冤袁抗体滴度更好袁52日龄咽喉拭子PCR检测R株阳性率免疫组低于对照组袁攻毒后免疫组对气囊保护率达99%袁而对照组均出现气囊病变遥本试验结果证实鸡毒支原体活疫苗渊F36株冤具备良好的安全性与有效性袁为该疫苗产品免疫效果评估提供数据参考遥关键词鸡毒支原体活疫苗渊F36株冤安全有效文献标识码院A文章编号院1003-4331渊2023冤04-0053-03EvaluationoftheimmuneefficacyofliveMycoplasmagallisepticumvaccine(F36strain)ZhangHuiyun渊JofunhwaBiotechnology(Fuzhou)Co.Ltd,Fuzhou350014冤AbstractTheinfectionrateofMycoplasmagallisepticumindomesticchickenfarmsishigh.vaccinationisoneoftheeffectivemeth鄄odstopreventandcontrolMG.ThepurposeofthisexperimentwastoobservethesafetyandeffectivenessofliveMGvaccine(F36strain)inimmunizingchickens.807-day-oldSPFchickenswithsimilarweightwereselected,theimmunizationgroupwasimmunizedwithliveMGvaccine(F36strain)andthecontrolgroupwasimmunizedwithphysiologicalsalinebythespot-eyeimmunity.Theimmu鄄nizedgroupandthecontrolgroupwerechallengedwithMycoplasmagallisepticumRstrainattheageof38days.Afterthechallenge,thefeedintake,mentalcondition,respiratorysymptomsanddeathofthechickenswereobservedeveryday.Thechickensofthetwogroupswereweighedattheageof38daysand52days,respectively.Bloodsampleswerecollectedat7days,14days,21days,38daysand52daysforantibodydetecti...
