第22卷第4期2023年8月现代农药ModernAgrochemicalsVol.22No.4Aug.2023·残留与环境·超高效液相色谱-串联质谱法同时测定草莓中咯菌腈和异菌脲残留量赵圣英1,高祥文1,于建垒²,刘同金²,梁慧²,房锋2*,李瑞娟2*(1.山东仕邦农化有限公司，济南250100;2.山东省农业科学院植物保护研究所，济南250100)摘要：为了建立一种同时检测草莓中咯菌和异菌脲残留量的分析方法，本研究采用乙睛提取草莓样品中的咯菌睛和异菌脲,GCB、PSA净化,超高效液相色谱-串联质谱检测，外标法定量。结果表明，咯菌睛和异菌脲的质量浓度与峰面积间在0.01～1mg/L范围内呈良好线性关系，咯菌睛在0.01~3.0mg/kg添加水平下，平均回收率为83%~105%，相对标准偏差为2.0%~12.1%；异菌脲在0.01~10mg/kg添加水平下，平均回收率为93%～101%，相对标准偏差为4.9%~9.4%，咯菌睛和异菌脲的最低检出浓度均为0.01mg/kg。该方法简单、灵敏度高、重现性好，适用于草莓样品中同时测定咯菌睛和异菌脲的残留量。关键词：咯菌睛；异菌脲；草莓；超高效液相色谱-串联质谱法；残留中图分类号：TQ450.2DeterminationofFludioxonilandIprodioneResiduesinStrawberrybyUPLC-MS/MSZHAOShengying',GAOXiangwen',YUJianlei,LIUTongjin²,LIANGHui,FANGFeng",LIRuijuan?*(1.ShandongShibangAgriculturalChemitryCo.,LTD,Jinan250100,China;2.InstituteofPlantProtection,ShandongAcademyofAgriculturalSciences,Jinan250100,China)Abstract:ThisstudyaimstodevelopanewmethodforresidueanalysisoffludioxonilandiprodioneinstrawberrybyUPLC-MS/MS.Fludioxonilandiprodioneresiduesinstrawberrywereextractedwithacetonitrile,purifiedbyGCBandPSA,anddeterminedbyUPLC-MS/MS.Thequantitativeanalysiswascarriedoutbyexternalstandardmethod.Theresultsshowedthatfludioxonilandiprodioneshowedagoodlinearrelationshipatthemassconcentrationrangeof0.01-1mg/L.Attheadditionlevelof0.01-3.0mg/kg,theaveragerecoveriesoffludioxonilinstrawberrywere83%-105%,therelativestandarddeviationswere2.0%-12.1%.Attheadditionlevelof0.01-10mg/kg,theaveragerecoveriesofiprodioneinstrawberrywere93%-101%,therelativestandarddeviationswere4.9%-9.4%.Thedetectionlimitoffludioxonilandiprodionewere0.01mg/kg.Themethodissimpleandreliable,sensitiveandreproducible,whichcanbeappliedfordeterminingfludioxo...
