·562·药品评价DrugEvaluation2023,20（5）HPLC法同时测定黄连上清片中栀子苷、黄芩苷的含量彭玲娜，孙辉，丁野，李文莉，赵桂铃湖南省药品检验检测研究院，湖南省药品质量评价工程技术中心，湖南长沙410001摘要：目的建立HPLC法，同时测定黄连上清片中黄芩苷和栀子苷的含量。方法以十八烷基键合硅胶为填充剂，0.2%磷酸溶液和乙腈为流动相进行梯度洗脱，流速为1.0mL/min，波长为250nm，柱温为35℃，进样量为10μL。结果栀子苷在1.971~985.6μg/mL（y=1003708.57x+33870.22，r=1.0000），黄芩苷在8.500~340.0μg/mL（y=1315348.69x+14934.22，r=1.0000）均呈良好的线性关系；平均回收率分别为96.67%、100.30%。结论含量测定方法操作简单，准确耐用，为黄芩、栀子药材在该制剂中的质量控制和标准改进提供了科学依据。关键词：高效液相色谱法；黄连上清片；栀子苷；黄芩苷DOI:10.19939/j.cnki.1672-2809.2023.05.10SimultaneousDeterminationofGeniposideandBaicalininHuanglianShangqingTabletsbyHPLCPENGLingna,SUNHui,DINGYe,LIWenli,ZHAOGuilingHunanInstituteofDrugInspectionandTesting,HunanEngineeringandTechnologyResearchCenterforPharmaceuticalQualityEvaluation,ChangshaHunan410001,China.Abstract:ObjectiveToestablishaHPLCmethodforsimultaneousdeterminationofgeniposideandbaicalininhuanglianshangqingtablets.MethodsItwastestedwithC18column,using0.2%phosphoricacidandacetonitrileforgradientelution,theflowratewas1.0mL/min,thewavelengthwas250nm,columntemperatureat35℃andtheinjectionvolumewas10μL.ResultsThetestresultsshowthatgeniposideandbaicalinallhadagoodlinearrelationshipintherangeof1.971~985.6μg/mL(y=1003708.57x+33870.22,r=1.0000)and8.500~340.0μg/mL(y=1315348.69x+14934.22,r=1.0000),respectively.Theaveragerecoveryrate(n=6)was96.67%and100.30%,respectively.ConclusionThemethodissimple,accurateanddurable,andprovideascientificbasisforthequalitycontrolandstandardimprovementofscutellariaeradixandgardeniaefructusinthispreparation.Keywords:Highperformanceliquidchromatography(HPLC);Huanglianshangqingtablets;Geniposide;Baicalin黄连上清片是由大黄、黄芩、黄柏、栀子等17味中药组成的复方制剂［1］。方中黄连、黄芩、黄柏、石膏清热泻火，燥湿解毒，栀子、大黄清热凉...
