1第35卷第2期2023年VOL.35NO.22023菏泽医学专科学校学报JOURNALOFHEZEMEDICALCOLLEGEDOI:10.3969/j.issn.1008-4118.2023.02.001*白藜芦醇/siRNA胶束复合物的载药和释药性能研究贾莉，苗方，张卫敏，安芸，董晓辉，马兰（菏泽医学专科学校，山东菏泽274000）摘要：目的研究pH和还原性双响应白藜芦醇/siRNA胶束复合物（MCpH/Redox@Res-siRNA）的载药和释药性能。方法采用紫外分光光度法和荧光分光光度法分别建立白藜芦醇（Resveratrol，Res）和FAM-siRNA体外分析方法，超速离心法和超滤离心法测定MCpH/Redox@Res-siRNA中药物的包封率（EncapsulationEfficiency，EE%）和载药量（DrugLoading，DL%），透析法测定MCpH/Redox@Res-siRNA中药物的释放性能。结果Res和FAM-siRNA的体外分析方法均符合方法学要求；MCpH/Redox@Res-siRNA中Res的EE%为（92.2±1.74）%、DL%为（9.72±1.63）%，FAM-siRNA的EE%为（94.5±1.42）%；MCpH/Redox@Res-siRNA中Res释放具有pH敏感性，siRNA的释放呈现pH和还原环境依赖性，在pH5.5和还原性环境中，Res和FAM-siRNA释放较为迅速和完全。结论MCpH/Redox@Res-siRNA具有较好的Res和siRNA载药性能和释放性能。关键词：胶束复合物；载药性能；释药性能中图分类号：R285文献标识码：A文章编号：1008-4118（2023）02-0001-04StudyondrugloadinganddrugreleasepropertiesofResveratrol/siRNAmicellarpolyplexesJIALi,MIAOFang,ZHANGWeimin,ANYun,DONGXiaohui,MALan（HezeMedicalCollege，Heze274000，Shandong）Abstract:ObjectiveTostudythedrugloadinganddrugreleasepropertiesofpHandreducingdouble-responsiveResveratrol/siRNAmicellarpolyplexes(MCpH/Redox@Res-siRNA).MethodsUVspectrophotometryandfluorescencespectrophotometrywereestablishedforResveratrol(Res)andFAM-siRNAinvitrorespectively.EncapsulationEfficiency(EE%)andDrugLoading(DL%)ofResandFAM-siRNAinMCpH/Redox@Res-siRNAweredeterminedbyultrafiltrationcentrifugalmethodandultrafiltrationcentrifugalmethod.ThedrugreleasepropertiesinMCpH/Redox@Res-siRNAwasdetectedbydialysis.ResultsTheEE%ofResandDLwere(92.2±1.74)%,(9.72±1.63)%inMCpH/Redox@Res-siRNA.TheEE%ofFAM-siRNAwere(94.5±1.42)%.ThereleaseofResinMCpH/Redox@Res-siRNAwaspH-responsive,andthereleaseofsiRNAispHandreducingenvironmentdependent.InpH5.5andreducingenvironme...
