·论著·NCOA5通过调控上皮间质转化促进卵巢癌细胞的侵袭转移能力*刘俊成洁庄珩之王圣坦刘晓行(海南省人民医院妇科,海南海口570311)【摘要】目的研究核受体共激活因子5(NCOA5)对卵巢癌细胞侵袭和转移能力的作用及分子机制。方法常规培养卵巢癌细胞株SKOV-3、A2780、OC3、HO-8910和正常卵巢上皮细胞系IOSE80,Westernblot检测各细胞中NCOA5蛋白的表达水平。选取高表达NCOA5的卵巢癌细胞SKOV-3和A2780,各分为si-NC组、si-NCOA5组,并分别转染NCsiRNA和NCOA5siRNA。Boyden实验检测各组细胞侵袭能力,Transwell实验检测各组细胞转移能力,Westernblot检测各组细胞上皮间质转(EMT)相关蛋白E-cadherin、N-cadherin、vimentin和Slug蛋白的表达水平。结果Westernblot结果显示NCOA5在卵巢癌细胞中的表达显著上调(P<0.05)。在SKOV-3和A2780细胞中干扰NCOA5的表达均显著抑制卵巢癌细胞侵袭和转移能力(P<0.05),并促进EMT相关蛋白E-cadherin的表达,抑制细胞中EMT相关蛋白N-cadherin、vimentin和Slug蛋白的表达(P<0.05)。结论NCOA5促进卵巢癌侵袭转移能力,具有作为卵巢癌新型预后生物标志物和治疗分子靶点的潜力。【关键词】卵巢癌;NCOA5;侵袭;转移;EMT【中图分类号】R737.31【文献标志码】ADOI:10.3969/j.issn.1672-3511.2023.09.007基金项目:海南省卫生计生行业科研项目(18A200114)通讯作者:王圣坦,E-mail:2000wangtan@163.com引用本文:刘俊,成洁,庄珩之,等.NCOA5通过调控上皮间质转化促进卵巢癌细胞的侵袭转移能力[J].西部医学,2023,35(9):1282-1286.DOI:10.3969/j.issn.1672-3511.2023.09.007NCOA5promotestheinvasionandmetastasisofovariancancercellsbyregulatingEpithelial-mesenchymaltransitionLIUJun,CHENGJie,ZHUANGHengzhi,WANGShengtan,LIUXiaoxing(DepartmentofGynaecology,HainanProvincialPeople'sHospital,Haikou570311,China)【Abstract】ObjectiveTostudytheroleofNuclearReceptorCoactivator5(NCOA5)onovariancancercellsandmolecularmechanisms.MethodsOvariancancercelllinesSKOV-3,A2780,OC3,HO-8910andnormalovarianepithe-lialcelllineIOSE80wereculturedregularly.WesternblotanalysiswasperformedtodetecttheexpressionlevelofNCOA5proteinineachcellline.OvariancancercellsSKOV-3andA2780withhighexpressionofNCOA5wereselectedanddividedintosi-NCgroupandsi-NCOA5group,respectively,andtransfectedwithNCsiRNAandNCOA5siRNA,respect...
