不同培曲工艺对清香型大曲细菌菌群结构的影响王军燕,甄攀,王晓勇(山西杏花村汾酒厂股份有限公司,山西汾阳032205)摘要:通过高通量测序技术对比分析了两种培曲工艺大曲的细菌菌群结构。结果表明,传统培曲工艺大曲Chao1指数和Shannon指数均较高,说明传统工艺曲细菌结构分布较新型工艺曲更均衡、菌群多样性更高。同时,传统工艺曲细菌菌群结构与新型工艺曲相比存在显著差异。传统工艺曲优势菌属为、、Bacillus,新型工艺曲优势菌属为、、。研究成果为清香型大曲的工艺改进、质量提升提供了理论依据。关键词:清香型大曲;培曲工艺;高通量测序;细菌菌群结构中图分类号:TS262.32;TS261.4;TS261.1文献标识码:BEffectsofDifferentDaquCultureTechniquesonBacterialFloraStructureofQingxiangDaquWANGJunyan,ZHENPan,WANGXiaoyong(ShanxiXinghuacunFenjiuDistillery,Fenyang032205,Shanxi,China)Abstract:ThisstudycomparingthebacterialflorastructureoftwokindsofDaquculturetechniquebyhigh-throughputsequencing.TheresultsshowedthatChao1andShannonindexoftraditionalculturetechniquesofDaquwerebothhigher,indicatingthatthebacterialflorastructuredistributionoftraditionalculturetechniquesofDaquwasmorebalancedandthebacterialdiversitywashigherthanthatofnewculturetechniquesofDaqu.Atthesametime,thereweresignificantdifferencesonbacterialflorastructureoftraditionalculturetechniquesofDaqucomparedwiththenewculturetechniquesofDaqu.ThetraditionalculturetechniquesofDaqudominantbacteriaare,and,whilethenewculturetechniquesofDaqudominantbacteriaare,and.TheresearchresultsprovidesatheoreticalfoundationfortechnologyandqualityimprovementofQingxiangDaqu.Keywords:QingxiangDaqu,Daquculturetechniques,High-throughputsequencing,Bacterialflorastructure曲是酒之骨。曲是白酒生产发酵的原动力,是白酒酿造的糖化剂和发酵剂,是白酒酿造的主要酶源[1-2]。清香型大曲采用大麦和豌豆制曲作为糖化发酵剂,依靠厂区环境带入的各种微生物,在淀粉质原料中进行富集、扩大培养,形成酿酒所需的各类微生物,再经过培养、贮藏而制得。传统培曲工艺采用地面培曲方式,依靠人工经验调整苇席覆盖、窗户启闭大小及时长、调整曲间距和层高,通过人工进行十多次不同方法翻曲,才能达到培曲过程中温湿度均衡性的目的。传统...