2023年第16期广东化工第50卷总第498期www.gdchem.com·19·不同NO3--N/NH4+-N配比对丹江口水库脆杆藻生长和叶绿素荧光的影响刘晓真,李园园,马甜莹(河南理工大学资源环境学院,河南焦作454003)[摘要]通过模拟丹江口水库总氮浓度1.5mg/L,以硝酸钠和氯化铵作为氮源,研究了硝铵配比为0∶100、25∶75、50∶50、75∶25、100∶0条件下脆杆藻细胞密度和叶绿素荧光参数的变化。结果表明:随着硝氮比例的增加,脆杆藻的藻细胞密度呈逐渐增高的趋势,且100∶0组的比生长速率(0.174)明显大于其他各组(p<0.05)。Fv/Fm、α、ETRmax值均表现出高比例硝氮组大于低比例硝氮组,与其生长趋势较为一致。研究表明,在1.5mg/L总氮浓度下提高硝氮比例可促进脆杆藻的生长以及提高其对光能利用效率。[关键词]脆杆藻;NO3--N/NH4+-N;生长特性;叶绿素荧光[中图分类号]X172[文献标识码]A[文章编号]1007-1865(2023)16-0019-03EffectsofDifferentNO3--N/NH4+-NontheGrowthandChlorophyllFluorescenceofFragilariasp.inDanjiangkouReservoirLiuXiaozhen,LiYuanyuan,MaTianying(InstituteofResourcesandEnvironment,HenanPolytechnicUniversity,Jiaozuo454003,China)Abstract:TheeffectsofdifferentNO3--N/NH4+-N(0∶100,25∶75,50∶50,75∶25,100∶0)onthecelldensityandchlorophyllfluorescenceparametersofFragilariatenuistipitatawerestudiedbysimulatingthetotalnitrogenconcentrationof1.5mg/LinDanjiangkouReservoirwithsodiumnitrateandammoniumchlorideasnitrogensources.Theresultsshowedthatwiththeincreaseofnitratenitrogenratio,thealgalcelldensityofbrittlerodalgaeincreasedgradually,andthespecificgrowthrateof100∶0group(0.174)wassignificantlyhigherthanthatofothergroups(p<0.05).Fv/Fm,αandETRmaxvaluesshowedthatthehighproportionofnitratenitrogengroupwasgreaterthanthelowproportionofnitratenitrogengroup,whichwasconsistentwithitsgrowthtrend.Studieshaveshownthatincreasingtheratioofnitratetonitrogenat1.5mg/Ltotalnitrogenconcentrationcanpromotethegrowthofbrittlerodalgaeandimproveitslightenergyutilizationefficiency.Keywords:Fragilariasp.;NO3--N/NH4+-N;growth;chlorophyllfluorescenceparameters浮游植物作为水体中主要的初级生产者,是水生态系统中最重要的组成部分,对保持水域生态系统平衡、物质循环与能量流动...