TianjinMedJ,September2023,Vol.51No.9丙泊酚麻醉损伤工作记忆编码海马-前额叶皮质网络信息传递的研究郭东勇1，李宝玲2，白文文2，刘迢迢2，徐新宇2△摘要：目的研究丙泊酚麻醉是否损伤大鼠工作记忆编码阶段海马到前额叶皮质神经通路的信息传递。方法12只大鼠中选取可用于研究分析的6只SD大鼠（3月龄），将16通道微电极阵列分别植入大鼠腹侧海马（vHPC）和内侧前额叶皮质（mPFC）。使用Cerebus信息采集系统记录每只大鼠在接受150mg/kg丙泊酚麻醉前，麻醉后12、24h执行工作记忆编码阶段任务时，其mPFC和vHPC这两个责任脑区的多通道局部场电位（LFPs）信号，建立vHPC-mFPC网络，分别计算vHPC和mPFC网络的定向传递函数（DTF）和vHPC-mPFC神经通路信息流，定量表征大鼠麻醉前后vHPC-mPFC神经通路信息传递。结果麻醉后12h大鼠vHPC和mPFC的平均网络连接强度及vHPC-mPFC信息流均低于麻醉前，而麻醉后24hvHPC和mPFC的平均网络连接强度及vHPC-mPFC信息流较麻醉前差异均无统计学意义。结论丙泊酚麻醉短暂损伤工作记忆编码vHPC和mPFC网络连接强度和vHPC-mPFC信息传递。关键词：二异丙酚；海马；大脑皮质；额叶；脑电描记术；工作记忆编码中图分类号：R318文献标志码：ADOI：10.11958/20230307AstudyofinformationtransmissioninHPC-PFCnetworkduringworkingmemoryencodinginducedbypropofolanesthesiaGUODongyong1,LIBaoling2,BAIWenwen2,LIUTiaotiao2,XUXinyu2△1DepartmentofAnesthesiology,TianjinMedicalUniversityCancerInstituteandHospital,Tianjin300060,China;2SchoolofBiomedicalEngineeringandTechnology,TianjinMedicalUniversity△CorrespondingAuthorE-mail:xuxinyu@tmu.edu.cnAbstract:ObjectiveTofigureoutwhetherpropofolanesthesiaimpairsinformationtransmissionfromhippocampaltoprefrontalcortex(HPC-PFC)neuralpathwaysduringthecodingphaseofworkingmemoryinrats.MethodsAmong12rats,6SDrats(3monthsold)wereselectedforresearchanalysis,and16-channelmicroelectrodearrayswereimplantedintothemedialprefrontalcortex(mPFC)andtheventralhippocampus(vHPC)ofrats,respectively.TheCerebusinformationacquisitionsystemwasusedtorecordtheworkingmemorycodingstagetasksofeachratbeforeand12and24hoursafteranesthesiawith150mg/kgpropofol.Themultichannellocalfieldpotential(LFPs)signalsofmPFCandvHPC,tworesponsiblebrainregions...
