第18卷第2期2023年6月贵阳学院学报（自然科学版）（季刊）JOURNALOFGUIYANGUNIVERSITYNaturalSciences(Quarterly)Vol.18No.2Jun.2023Box－behnken响应面法优选肾风止痒方提取工艺研究熊成欢1²2,曹佩雪3,屈相玲12*，陈礼大",张雪，张方文（1.贵州中医药大学第二附属医院药学部，贵州贵阳550001；2.医院制剂研发中心，贵州贵阳550001；3.贵州省中国科学院天然产物化学重点实验室，贵州贵阳550014)摘要：以没食子酸和盐酸小檗碱含量的综合评分为评价指标，在单因素实验基础上，采用Box-Behnken响应面法考察肾风止痒方最佳提取工艺。最佳提取工艺为：75%乙醇为溶媒，液料比12:1（mL/g），常温浸泡96小时。验证试验综合评分95.02，与优选工艺相对误差为0.19%。本法简单，预测性良好，提取工艺稳定，为肾风止痒方的研究开发奠定基础。关键词：肾风止痒方；响应面法；工艺优化中图分类号：R284.2OptimizationoftheextractionprocessofcompoundpreparationofShenFengZhiYangbyBox-behnkenDesign-responseSurfaceXIONGCheng-huan'-CAOPei-xue',QUXiang-ling'-2,ChenLi-da,ZHANGXue',ZHANGFang-wen'(1.Dept.OfPharmacy,theSecondAffiliatedHospitalofGuizhouUniversityofTraditionalChineseMedicine,Guiyang550001,Guizhou,China;2.HospitalPreparationResearch&DevelopmentCentre,Guiyang550001,Guizhou,China;3.TheKeyLaboratoryofChemistryforNaturalProductsofGuizhouProvinceandChineseAbstract:Takingthecontentsofgallicacidandberberinehydrochlorideasresponsevalues,Onthebasisofsingle-fac-torexperiments,Box-BehnkenresponsesurfacemethodwasusedtopredictthebestalcoholextractionprocessofShen-fengZhiyangRecipe.Theoptimalconditionswereasfllows:ethanolconcentrationof85%,liquid-solidratioof12.5:1(mL/g),extractiontimeof4.60daysatroomtemperature.Itwasdemonstsratedthattheaveragecomprehensivescorewas95.02bytheverifiedexperient.Therelativeerrorwas0.19%comparedwiththepredictedvalue.Theextrac-tionprocessissimple,stableandpredictable,whichlaysthefoundationfortheresearchanddevelopmentofShenfengZhiyangRecipe.Keywords:ShenFengZhiYangpreparation;Centralcompositedesign-responsesurfacemethodology;Processoptimi-zation肾风止痒方是由五倍子、黄柏、地肤子等中药组成的临床验方，具有清热利湿、祛风止痒功效，...
