第52卷第2期2023年6月发酵科技通讯BulletinofFermentationScienceandTechnologyVol.52No.2Jun.2023超声辅助提取玉米黄色素及其抗氧化活性研究马丽媛'",石光蕊",尚尔坤”,黄雪莹",张晟",李杨",郭丽",王喜庆!(1.绥化学院食品与制药工程学院,黑龙江绥化152061;2.绥化市检验检测中心,黑龙江绥化152000)摘要:以玉米加工副产品玉米皮渣为原料,利用超声波辅助醇提取法提取玉米黄色素。以玉米黄色素的提取率为主要衡量指标,通过单因素实验和正交实验,得到提取玉米黄色素的最佳工艺条件。正交实验结果表明:当超声浸提时间为40min、料液比为1:12g/mL、提取温度为60℃、溶液pH为6时,玉米黄色素最高提取率为6.02%。抗氧化实验结果表明:当样品质量浓度为1,2,3,4,5mg/mL时,提取的玉米黄色素具有一定的总还原能力、清除DPPH自由基和羟基自由基能力,且这些能力随着样品质量浓度的增加而提高。超声法提取玉米黄色素提取率高,所提取的玉米黄色素具有一定的抗氧化能力。关键词:玉米黄色素;超声波;提取率;玉米皮渣;抗氧化活性中图分类号:TQ920Studyonultrasound-assistedextractionofcornyellowpigment文献标志码Aanditsantioxidantactivity文章编号:1674-2214(2023)02-0063-06MALiyuan',SHIGuangrui',SHANGErkun’,HUANGXueying',ZHANGSheng',LIYang',GUOLi',WANGXiqing'(1.SchoolofFoodandPharmaceuticalEngineering,SuihuaUniversity,Suihua152061,China;2.SuihuaInspectionandTestingCenter,Suihua152000,China)Abstract:Cornhuskresiduefromtheby-productofcornprocessingwasusedastherawmaterialforultrasonic-assistedethanolextractionofcornyellowpigment.Theextractionrateofcornyellowpigmentwasselectedastheprimarymeasurementindexbasedonasinglefactorandorthogonalexperimentsinordertodeterminetheoptimalextractionconditions.Whentheultrasonicextractiontimewas40min,thematerial-to-liquidratiowas1:12g/mL,theextractiontemperaturewas6oC,andthepHvalueofthesolutionwas6,thehighestextractionratewas6.02%.Theantioxidantactivitytestrevealedthattheextractedcornyellowpigmentpossessedacertaintotalreducingability,DPPHandhydroxylfreeradicalscavengingability,andthatthisabilityincreasedwiththesampleconcentration,whenthesampleconcentrationwas1~5mg/mL.Itdemonstratedthatthepigment'sextractionra...