农产品加工2022年第12期摘要:以克氏原鳌虾虾头为原料,通过碱性蛋白酶酶解制备虾头酶解液,以水解度为评价指标,探讨酶添加量、酶解时间、酶解温度、酶解pH值、料液比对酶解效果的影响。通过单因素试验及响应面法确定最佳酶解条件为碱性蛋白酶添加量2000U/g,料液比1∶1.2,pH值8.92,酶解时间3.11h,酶解温度60.66℃,在此条件下,蛋白质水解度达到最大值,为25.31%。关键词:克氏原螯虾;虾头;酶解;响应面法;水解度中图分类号:TS254.9文献标志码:Adoi:10.16693/j.cnki.1671-9646(X).2022.12.035OptimizationofEnzymaticHydrolysisofProcambarusclarkiiHeadbyResponseSurfaceMethodologyYANYuhan1,2,*LUJingjing1(1.CollegeofFood&BiologyScienceandTechnology,WuhanInstituteofDesignandSciences,Wuhan,Hubei430205,China;2.CollegeofFoodandBioengineering,JiangsuUniversity,Zhenjian,Jiangsu212023,China)Abstract:Inthisstudy,theProcambarusclarkiiheadwasusedasrawmaterialtopreparetheenzymatichydrolysissolutionofshrimpheadbyalkalineprotease.Thedegreeofhydrolysiswasusedastheevaluationindextoinvestigatetheeffectsofenzymeadditionamount,enzymatichydrolysistime,enzymatichydrolysistemperature,enzymatichydrolysispH,andsolid-liquidratioontheenzymatichydrolysiseffect.Accordingtothesinglefactorandresponsesurface,theoptimalenzymatichydrolysisconditionswerealkalineproteasedosageof2000U/g,solid-liquidratioof1∶1.2,pH8.92,enzymatichydrolysistimeof3.11h,andenzymatichydrolysistemperatureof60.66℃.Undertheseconditions,thedegreeofproteolysisreachedthemax-imumof25.31%.Keywords:Procamborusclarkii;shrimphead;enzymolysis;responsesrufacemethod;degreeofhycholysis响应面法优化克氏原螯虾虾头酶解工艺研究燕瑜翰1,2,*卢静静1(1.武汉设计工程学院食品与生物科技学院,湖北武汉430205;2.江苏大学食品与生物工程学院,江苏镇江212023)收稿日期:2022-08-31作者简介:燕瑜翰(2000—),女,在读硕士,研究方向为粮油食品加工。*通讯作者:卢静静(1987—),女,硕士,讲师,研究方向为粮油食品加工。0引言克氏原螯虾因其味道鲜美、蛋白质含量丰富、营养价值高、烹饪方式多样,受到广大消费者的青睐。在克氏原螯虾加工和消费过程中,产生了大量的虾头、虾...