ICS65.020.01B40DB37山东省地方标准DB37/T3533—2019驴骡马皮张源性成分鉴定实时荧光定性PCR法Identificationofdonkey,mule/hinnyandhorsederivedmaterialsinleatherReal-timefluorescentPCRmethod2019-03-21发布2019-04-21实施山东省市场监督管理局发布DB37/T3533—2019I目次前言................................................................................II1范围...............................................................................12规范性引用文件.....................................................................13术语和定义.........................................................................14原理...............................................................................15试剂或材料.........................................................................15.1试剂...........................................................................15.2材料...........................................................................25.3配制溶液.......................................................................26仪器设备...........................................................................37检测用引物和探针...................................................................38试验步骤...........................................................................38.1样本制备.......................................................................38.2DNA提取........................................................................38.3实时荧光PCR反应...............................................................39试验数据处理.......................................................................49.1PCR有效性判定..................................................................49.2检测结果分析和表述.............................................................410检测过程中防止交叉污染的措施......................................................4附录A（规范性附录）实时荧光定性PCR引物/探针序列、反应体系和结果判定...........5附录B（资料性附录）目的基因扩增靶标参考序列.......................
