IdentificationofculturedadultratRGCsIdentificationofculturedadultratRGCs.Culturedcells.Culturedcellswereco-labeledwithanti-Thy-1antibody(A),anti-NF-Lwereco-labeledwithanti-Thy-1antibody(A),anti-NF-Lantibody(B),andDAPI(C).(D)Adigitallymergedimageantibody(B),andDAPI(C).(D)Adigitallymergedimagesimultaneouslyrepresentsallthreefluorescentlabels.(E)Thesimultaneouslyrepresentsallthreefluorescentlabels.(E)Thecorrespondingphase-contrastimage.correspondingphase-contrastimage.免疫荧光染色法免疫荧光染色法用于标记二抗体的荧光素：用于标记二抗体的荧光素：（（11）异硫氰酸荧光素（）异硫氰酸荧光素（FITCFITC））发射的荧光波长为发射的荧光波长为490490～～619619（绿色...
