ProtocolofthepolymerasechainreactionfordetectinggeneticallymodifiedcomponentsinrapeseedsI~;~~&S~~~HM%VM.%@TMEOB~,&B~B~C:%B~~$I%#~W.I~~~#&PCR@WA&.*6@SHT&9ttNiPCDfBl,%tTMR@&4.@B;l;Bff%!fdJle##fsel@BPCR&MeTPI*%.5!XW@B8BSHT*4%?Bo3.1t)Stransgene~~#;Q~if~a~,*a~~i~~~marnDNAB~I.~~&N+A+H,@~&BP~#+~Ei-S.Q1Yi,U@BalfJbR4~8ii#611jPP%~e32fir&L#*K&polymerasechainreaction,PCW#&SE4@3'15k&&S2f!k&%J%6%,fiDNA#$B#H#SaMEj&%#T,&B#&%9JRit~R&Cll$hJJ)41]-4#&DNAm&@k#flff~-BIjllj:.@3ilJ%%aIkThi&X@&DNASe~H#HTU~#~&BM~~(dN~~')%E~~@9I~l9U~i~*#G5~BB3~~~-m*,@BrMmsm%Bunfi%arme33a#i%3.3.1PCR;polymerescchainreact~on,iQ-%PCR.3.3.2DNArdeoxyribonucleic~cid,a#%&#l&@~3.3.3dNTP;deoxyribonucleos~detripl1osi>hate,A#R&BBZbf9@.3.3.4dATP,deoxyadenos~netriphosphatc,8#$lMfi7@i!#ie3.3.5dCTPIdeoxycytid~netr~phosphnte,H##H%~.ljlPaQ.3.3.6dCTPtdeoxyguanos~netr1phos~hatc,A#$l4%3@@.3.3.7dTTP;deoxythymidinetriphospllate,A#~!jQH#:a&(ft.3.38dUTPldeoxyuridinetripho~phatc,R#$t@-ff'Z7#@!~3.3.9UDGIuracilDNAglycosylase,!3!@/@DNA-%#@.3.3.10bpIbasepair,@&XQ.33.11EDTAleth~lenediaminetetraaceticacid,&f@fl&@41.3.3.12TagITaqDNAPolymerase,DNASeIBB),3.3.13TEITris-HC1,EDTA$@#%.3.3.14SDS~sodiumdodecyls~lfate,+=~~~@~~3.3.15Tris;tris(hydroxymethyl)aminomethanevZ(BtB#)$2&$;Sm3.3.16PEPIpho~phoenolpyruvatecarboxylasegene,?i@l@#lBSWRm%.cI:@.33.17FMV35S135Spromoterfromamodifiedfigwortmosaicvirus(caulimovirusgroup),-k;$aRt.%f*35sB33.3.3.18CP4EPSPSl5-enolpyruvylshikimate3-phosphatesynthasegene,5-%Q:@-3-@@fi&&€%I.33.19CTPIchloroplasttransitpeptidegene,Utb3#BM!lkSF4a3.3.20G0X:glyphosateoxidoreductasegenerWE8P)tSfi%RWSH,33.21E93',3'sequenceofsmallsubunitofrbcS(ribu10se-I,5-bisphosphatecarboxylase)E9gene(frompea),S@T]BPtlXb9tlGH4$-1,5=RBB%.cI:BE9SB/J\YE2&3'MBY!!.3.3.22CaMV35S135Spromoterfromcauliflowermosaicvirus,a##%$E,Rt.#jSj35Sm23;bTJ..3.3.23PATIphosphinothricinacetyltransferasegenefromBacillusamyloliquefaciens.SHTRRBacillusamyloliquefaciensJ$JR#Zi%$$&i%S~.3.3.24BARNASE,ribonucleasegenefromBacillusumyloliquefaciens,MTRBacillusarnyloiiquefaciensrn...
