农业环境科学学报JournalofAgro⁃EnvironmentScience2023，42（6）:1294-13042023年6月景秀清，赵鹏宇，白雪，等.谷田土壤中细菌群落对铬胁迫的响应特征[J].农业环境科学学报,2023,42（6）：1294-1304.JINGXQ,ZHAOPY,BAIX,etal.Responsecharacteristicsofbacterialcommunitiestochromiumstressinthesoilofmilletfields[J].JournalofAgro-EnvironmentScience,2023,42（6）：1294-1304.谷田土壤中细菌群落对铬胁迫的响应特征景秀清1，2，赵鹏宇1*，白雪1，邓宁1，赵晓东1，吴丽华1，仪慧兰2（1.太原师范学院生物科学与技术学院，山西晋中030619；2.山西大学生命科学学院，太原030006）ResponsecharacteristicsofbacterialcommunitiestochromiumstressinthesoilofmilletfieldsJINGXiuqing1,2,ZHAOPengyu1*,BAIXue1,DENGNing1,ZHAOXiaodong1,WULihua1,YIHuilan2（1.CollegeofBiologicalSciencesandTechnology,TaiyuanNormalUniversity,Jinzhong030619,China;2.CollegeofLifeScience,ShanxiUniversity,Taiyuan030006,China）Abstract：Toinvestigatetheresponsemechanismofbacterialcommunitiesinfarmlandsoiltochromium（Cr）stress,soilplantedwith“Jingu21”millet（Setariaitalica）wasusedastheexperimentalmaterialinthisstudy.SampleswereacquiredbeforeCrstress（CKgroup）andafter6h（Cr_6h）and6d（Cr_6d）of1mmol·L−1Cr6+stress.Thecomposition,diversity,assemblyprocess,andKEGGmetabolicpathwayactivityofbacterialcommunityinthesoilunderCrstresswereanalyzedusingIlluminaMiSeqhigh-throughputsequencing.TheresultsrevealedthatthereweresignificantdifferencesintheproportionofdominantbacteriaatphylumandgenuslevelsinthebacterialcommunitiesunderdifferentdurationsofCrstress.Theαdiversityvariedinstages,forexampletheShannonindexdecreasedatCr_6handincreasedatCr_6d（6.07atCK,5.92atCr_6h,and6.04atCr_6d）,whereastheSimpsonindexexhibitedtheoppositetrend（0.0068atCK,0.0078atCr_6h,and0.0068atCr_6d）.AfterCrstress,thebetaNTIindexcontinuedtodecrease（−2.68atCK,−2.11atCr_6h,and−1.91atCr_6d）.ThebacterialcommunityassemblyprocessesinCKandCr_6hstageweredominatedbydeterministicassemblyprocess（||betaNTI>2）,whereasinCr_6dstage,theyweremainlydominatedbystochasticassemblyprocess（||betaN...
