茶叶通讯第50卷第1期JournalofTeaCommunicationVol.50,No.1投稿平台:http://cytx.cbpt.cnki.net褐藻寡糖对茶树抗寒性影响的研究赵子昕1,赵丽2,惠海滨3,侯剑3,范斌2,谢雪迎4*,韩晓阳1*1.山东农业大学园艺科学与工程学院,山东泰安271000;2.山东洁晶集团股份有限公司,山东日照276800;3.日照市农业技术服务中心,山东日照276800;4.齐鲁工业大学生态研究所/应用微生物学山东省重点实验室,山东济南250000摘要:为探究褐藻寡糖对茶树抗寒性的影响,以茶树品种‘福鼎大白茶’为试材,在低温胁迫下进行褐藻寡糖最适分子量范围和最适浓度试验,并以最优条件喷施茶苗,随后测定其在低温胁迫下的生理、生化及分子指标。结果表明,最适宜的褐藻寡糖分子量范围在1000~2000Da、质量浓度为1.0%且在低温胁迫前施用效果最佳。褐藻寡糖能够缓解叶片叶绿素的降解和水分的散失,提高叶片中SOD、CAT、POD等抗氧化酶活性和可溶性糖含量,增强CsCAT、CsGluTR、CsChls、CsSPS、CsCu/Zn-SOD、CsSUS4、CsCBF、CsICE及CsCOR等基因在叶片中的表达量。关键词:茶树;褐藻寡糖;低温胁迫;抗寒性中图分类号:S571.1文献标识码:A文章编号:1009-525X(2023)01-53-60EffectsofAlgalOligosaccharidesonColdResistanceofTeaPlantsZHAOZixin1,ZHAOLi2,HUIHaibin3,HOUJian3,FANBin2,XIEXueying4*,HANXiaoyang1*1.CollegeofHorticultureScienceandEngineering,ShandongAgricultureUniversity,Tai'an271000,China;2.ShandongJiejingGroupCo.,Ltd.,Rizhao276800,China;3.RizhaoAgriculturalTechnologyServiceCenter,Rizhao276800,China;4.ShandongProvincialKeyLaboratoryofAppliedMicrobiology/EcologyInstitute,QiluUniversityofTechnology(ShandongAcademyofSciences),Jinan250000,ChinaAbstract:Inordertoexploretheeffectofalginateoligosaccharidesonthecoldresistanceofteaplants,‘Fudingdabaicha’wasusedasthetestmaterialinthisexperiment.Theoptimummolecularweightrangeandoptimumconcentrationofalginateoligosaccharidesweretestedunderlowtemperaturestress.Thetheteaseedlingsweresprayedundertheoptimalconditions,andthenthephysiological,biochemicalandmolecularindexesweremeasuredunderlowtemperaturestress.Theresultsshowedthatthemolecularweightrangeofalginateoligosaccharidewas1000~2000Da,themassconcentrationwas1.0%.Theeffectofap...