2023年第6期广东化工第50卷总第488期www.gdchem.com·181·肤鲜洗剂质量标准研究邹戬，孙晓静*，张义生(武汉市中医医院综合药学部，湖北武汉430014)[摘要]目的：建立肤鲜洗剂的质量标准。方法：采用薄层色谱法(TLC)对肤鲜洗剂中地肤子、苦参、野菊花、白鲜皮进行定性鉴别；采用反向高效液相色谱法对黄柏中黄柏碱进行含量测定，色谱柱为E1921312SinoChromODS-BP5um(4.6×250mm)，流动相为乙腈-0.1%磷酸溶液(每100mL加十二烷基磺酸钠0.2g)(36∶64)，流速为1.0mL/min，检测波长为284nm，柱温为30℃。结果：TLC斑点清晰，分离度良好，阴性对照无干扰；黄柏碱在进样量范围内与峰面积呈良好线性关系，相关系数均在0.9999以上，平均加样回收率为99.2%，RSD为0.57(n=6)。结论：该方法专属性较强，且快速、准确、可以作为肤鲜洗剂的质量控制标准。[关键词]肤鲜洗剂；质量标准；薄层鉴别；黄柏碱[中图分类号]TQ[文献标识码]A[文章编号]1007-1865(2023)06-0181-03QualityStandardofFuxianLotionZouJian,SunXiaojing*,ZhangYisheng(WuhanHospitalofTraditionalChineseMedicine,GeneralPharmaceuticalDepartment,Wuhan430014,China)Abstract:Objective:ToestablishthequalitystandardofFuxianLotion.Methods:TLCwasusedtoqualitativelyidentifyRadixScutellariae,RadixSophoraeFlavescentis,ChrysanthemumindicumandCortexDictamniinFuxianLotion,andRP-HPLCwasusedtodeterminethecontentofPhellodendrineinPhellodendronPhellodendri.ThechromatographiccolumnwasE1921312SinoChromODS-BP5um(4.6×250mm),andthemobilephasewasacetonitrile-0.1%phosphoricacidsolution(every100mLwithsodiumdodecylsulfonate0.2g)(36∶64),flowrateof1.0mLmin-1,detectionwavelengthof284nm,columntemperatureof30℃.Results:TLCspotswereclear,theseparationwasgood,andthenegativecontrolhadnointerference.Phellodendrineshowedagoodlinearrelationshipwithpeakareaintherangeofsamplevolume,thecorrelationcoefficientswereabove0.9999,theaveragerecoveryratewas99.2%,RSDwas0.57(n=6).Conclusion:Themethodisspecific,fastandaccurate,andcanbeusedasthequalitycontrolstandardforFuxianLotion.Keywords:FuXianLotion；qualitystandard；TLCidentification；phellodendrine肤鲜洗剂为武汉市中医医院皮肤科外用洗剂，由地肤子、白鲜皮、黄柏、苦参、野菊花等九味中药组成...
