Univ.Chem.2023,38(1),187–191187收稿：2022-02-12；录用：2022-03-24；网络发表：2022-04-08*通讯作者，Email:cesxty@mail.sysu.edu.cn基金资助：中山大学实验教学研究(改革)项目•化学实验•doi:10.3866/PKU.DXHX202202025www.dxhx.pku.edu.cn手性毛细管电泳实验中手性选择剂取代度的影响研究谢天尧*，邓爱玲，肖华，朱芳中山大学化学学院，广州510275摘要：针对手性毛细管电泳实验“手性药物氧氟沙星对映体的分离检测”的教学过程中，发现手性选择剂取代度对实验结果具有重要影响，详细研究了一系列不同取代度的羟丙基-β-环糊精对氧氟沙星对映体分离效果的影响。研究结果表明：对映体的分离度随取代度变化显著，且取代度DS=7.9时分离度最佳。依据取代度的测定方法，对市售羟丙基-β-环糊精的质量进行评估，以确保手性毛细管电泳实验结果的稳定性和良好的教学效果。关键词：手性毛细管电泳实验；取代度；手性药物分析；羟丙基-β-环糊精；氧氟沙星；仪器分析实验中图分类号：G64；O657StudyontheInfluenceoftheSubstitutionDegreeofHydroxypropyl-β-CyclodextrininChiralCapillaryElectrophoresisExperimentsTianyaoXie*,AilingDeng,HuaXiao,FangZhuSchoolofChemistry,SunYat-SenUniversity,Guangzhou510275,ChinaAbstract:Becauseoftheimportantinfluenceofthechiralselectorsubstitutiondegreeontheenantioseparationofofloxacininchiralcapillaryelectrophoresisexperiments,theeffectsofthesubstitutiondegreeofhydroxypropyl-β-cyclodextrin(HP-β-CD)ontheinclusioncomplexbetweenofloxacinandHP-β-CDwereinvestigatedaccordingtodifferentsubstitutiondegrees.Theresultsshowedthattheenantioseparationresolutionchangedsignificantlywiththesubstitutiondegree.Moreover,thebestseparationresolutionwasfoundforaDS(degreeofsubstitution)=7.9.ThemethodsofdeterminingDSwerealsodiscussed.Usingthesemethods,thequalityofcommercialhydroxypropyl-β-cyclodextrinproductscanbeeffectivelymonitored,andthestabilityandeffectivenessinteachingofthechiralcapillaryelectrophoresisexperimentcanbeensured.KeyWords:Chiralcapillaryelectrophoresisexperiment;Substitutiondegree;Chiraldrugsanalysis;Hydroxypropyl-β-cyclodextrin;Ofloxacin;Instrumentalanalysisexperiment当药物分子结构含有手性中心时，由于空间构型的不同...
