农业环境科学学报JournalofAgro⁃EnvironmentScience2022，41（12）:2604-26122022年12月王莹，林凤翔，岳星雨，等.生物炭固定SM3菌剂的制备及其对三唑酮的降解研究[J].农业环境科学学报,2022,41（12）：2604-2612.WANGY,LINFX,YUEXY,etal.PreparationofbiocharimmobilizedSM3bacterialagentanddegradationoftriadimefon[J].JournalofAgro-EnvironmentScience,2022,41（12）：2604-2612.开放科学OSID生物炭固定SM3菌剂的制备及其对三唑酮的降解研究王莹，林凤翔，岳星雨，张赛，吴宗彬，施艳红*（安徽农业大学资源与环境学院，合肥230036）PreparationofbiocharimmobilizedSM3bacterialagentanddegradationoftriadimefonWANGYing,LINFengxiang,YUEXingyu,ZHANGSai,WUZongbin,SHIYanhong*（SchoolofResourcesandEnvironment,AnhuiAgriculturalUniversity,Hefei230036,China）Abstract：Toaddresstheproblemoftriadimefoncontaminationintheenvironment,thisstudypreparedanimmobilizedbacterialagentbyadsorptionmethodusingbiocharasacarrierandanefficienttriazolonedegradingbacteriumStenotrophomonasmaltophilia（SM3）asanimmobilizedstrain.Theimmobilizedcarrierswithcharacteristicsoflargespecificsurfaceareaandgoodadsorptionperformancewerepreferablyselectedfromfourtypesofbiochar,andtheirpreparationconditionswereoptimized,basedonwhichthestabilityofbiocharimmobilizedbacterialagentsandtheirdegradationeffectsontriazolonewereevaluated.Theresultsshowedthatamongthesefour,Camelliaoleiferabiocharhadlargespecificsurfaceareaandporespace,richfunctionalgroups,andbetterdegradationeffectofimmobilizedbacterialagentontriadimefonthantheotherthree,andhencewasselectedasthecarrierforimmobilizationofSM3bacteria.Thesingle-factoroptimizationtestshowedthatthedegradationrateoftriadimefonbytheimmobilizedbacterialagentreached93.27%whenpreparedatacarrierdosageof20mg·mL-1,SM3inoculumof5%,immobilizationtemperatureof30℃,andimmobilizationtimeof36h.Thedegradationrateoftheimmobilizedbacterialagentpreparedundertheoptimalconditionswasreportedreaching81.73%and58.18%fortriadimefonafter28dat4℃,andatroomtemperatures（25-35℃）,withthebacterialagentdisplayinggoodstability.Thepreparati...
