研究报告ResearchReport棉花GhGGB基因的克隆与表达分析玛迪娜·木拉提孙婷婷代培红王倩胡子曜徐诗佳刘超李月*新疆农业大学农学院,乌鲁木齐,830052*通信作者,liyue6905@126.com摘要蛋白异戊二烯化修饰与植物的抗旱性有关，GGB作为Ⅰ型香叶酰基转移酶的β亚基参与植物的抗逆途径。本研究以‘TM-1’棉花叶片的cDNA为模板，PCR克隆得到GhGGB基因，其开放阅读框为1131bp，编码377个氨基酸，为亲水性蛋白。此外，通过RT-qPCR技术检测和分析了GhGGB在不同棉花抗旱品种中根、茎、叶中的表达模式，以及GhGGB基因对不同胁迫处理的响应。结果表明GhGGB基因在不同抗旱性的棉花品种中无特异性表达；在相同培养条件下，GhGGB基因受不同胁迫处理后其表达量存在部分差异，其中对干旱处理尤为显著。随干旱胁迫时间的延长，GhGGB基因的表达量逐渐上升。本研究结果为解析棉花的抗逆分子机制提供了基础。关键词棉花;GGB基因;蛋白异戊二烯化修饰CloningandExpressionAnalysisofGhGGBGeneinCottonMadina·MulatiSunTingtingDaiPeihongWangQianHuZiyaoXuShijiaLiuChaoLiYue*CollegeofAgriculture,XinjiangAgriculturalUniversity,Urumqi,830052*Correspondingauthor,liyue6905@126.comDOI:10.13271j.mpb.021.000729AbstractProteinisoprenemodifyrelatedtodroughtresistanceofplant,GGBasⅠgeranylacyltransferasebetasubunitsalsoparticipatedintheartwayintheplant.Inthisstudy,using'TM-1'cottonleafcDNAastemplate,GhGGBgenewasclonedbyPCR.Itsopenreadingframewas1131bp,encoding377aminoacids,anditwasahy-drophilicprotein.Inaddition,RT-qPCRwasusedtoanalyzetheexpressionpatternsofGhGGBinroots,stemsandleavesofdifferentdrought-resistantcottonvarieties,aswellastheresponseofGhGGBgenetodifferentstresstreatmentsunderthesamecultureconditions.TheresultsshowedthatGhGGBgenehadnospecificexpressionincottonvarietieswithdifferentdroughtresistance.Underthesamecultureconditions,theexpressionlevelofGhG-GBgenewaspartiallydifferentafterdifferentstresstreatments,especiallyfordroughttreatment,andtheexpres-sionlevelgraduallyincreasedwiththeextensionofdroughtstresstime.Theresultsofthisstudyprovideabasisforthemolecularmechanismofstressresistanceincotton.KeywordsCotton;GGBgene;Proteinisoprenemodification基金项目：本研究由新疆...
