12中国果树,2023(3):12-17本文于2022-08-24收到,2022-11-09收到修改稿。*山东省果品产业技术体系项目(SDAIT-06-19)。吕毅电话:18963120868,E-mail:lvyiweihai@126.com。试验研究DOI:10.16626/j.cnki.issn1000-8047.2023.03.003连作对平邑甜茶幼苗叶片光系统Ⅱ、活性氧及抗氧化酶活性的影响*吕毅(威海市农业科学院,山东264200)摘要以盆栽平邑甜茶(Malushupehensis)幼苗为试材,探讨连作对叶片光系统功能的伤害机制及对活性氧含量、抗氧化酶活性的影响。结果表明,连作条件下,平邑甜茶幼苗生物量减少,叶片净光合速率降低,PSⅡ受到损伤,其响应机制是:PSⅡ供体侧放氧复合体受到损伤,QA被过度还原,光合电子传递链中的QA-大量积累,PSⅡ反应中心和受体侧均受到显著性伤害,受体侧由QA向QB的传递受阻,电子传递链受到损害,大量能量积累导致光能过剩及活性氧浓度上升,对光系统造成氧化破坏,抑制光合作用。同时,以吸收光能为基础的性能指数降低,实际光化学效率减弱,PSⅡ激发能分配方式发生改变,植株叶片通过增加热耗散等消耗过多激发能来适应连作带来的胁迫环境。连作条件下,平邑甜茶幼苗叶片抗氧化酶系统与活性氧之间动态平衡被打破,加剧了脂膜过氧化程度,加重了对叶片的伤害。关键词连作;平邑甜茶;幼苗;光系统Ⅱ;活性氧;抗氧化酶中图分类号:S661.1文献标志码:A文章编号:1000-8047(2023)03-0012-06StressofcontinuouscroppingonPSⅡ,reactiveoxygenspeciesandprotectiveenzymesactivityofMalushupehensisRehd.seedlingsLÜYi(WeihaiAcademyofAgriculturalSciences,Shandong264200)AAbstractTheMalushupehensisRehd.seedlingswereselectedasthetestedmaterials.Thepurposeofthisstudywastoinvestigatetheeffectsofcontinuouscroppingstressonphotosyntheticactivitiesandreactiveoxygenspeciesandprotectiveenzymesactivities.Theresultsshowedthatundercontinuouscroppingconditions,thebiomassofMalushupehensisRehd.seedlingsdecreased,thenetphotosyntheticrateofleavesdecreased,andthePSⅡwasdamaged.Theresponsemechanismwasthattheoxygen-evolvingcomplex(OEC)ofthePSⅡwasdamaged,QAwasover-reduced,QA-inthephotosyntheticelectrontransferchainwasaccumulated,thereactioncenterandacceptersideofPSⅡweresignificantlydamaged,thetransferfromQAtoQBattheacceptersidewasblocked,andtheelectrontransf...