※安全检测食品科学2023,Vol.44,No.04321基于香气指纹图谱和多元化学计量法对黄金茶2号等级的判别分析巢瑾1,2,周令欣2,银飞燕2,罗茜2,赵萌萌2,袁勇2,吴浩人2,李跑1,蒋立文1,*(1.湖南农业大学食品科学技术学院,湖南长沙410128;2.国家茶叶加工技术研发分中心,湖南省茶叶种植与加工工程技术研究中心,湖南省茶业集团股份有限公司,湖南长沙410126)摘要:以黄金茶2号为研究对象,采用顶空固相微萃取-气相色谱-质谱联用技术建立不同等级黄金茶香气成分气相色谱-质谱指纹图谱,并通过化学计量法对两个等级的黄金茶进行判别分析。研究表明,2个等级的黄金茶中鉴定出81种香气组分,其中41种为共有香气组分。基于共有香气组分的正交偏最小二乘判别分析可以区分2个等级黄金茶,并筛选出9种差异香气组分,分别是庚醛、柠檬烯、反式-β-罗勒烯、1-辛醇、反式-4,8-二甲基-1,3,7-壬三烯、反-橙花叔醇、1,10-diepicubenol、epicubenol、十六酸甲酯。以9种差异香气组分为变量建立的线性判别方程对2个等级的黄金茶进行等级判别,自身验证和交叉验证正确率为100%。本实验结果可为黄金茶的分级提供客观量化的参考依据。关键词:黄金茶;顶空固相微萃取;香气指纹图谱;等级判别;化学计量学GradeIdentificationofHuangjinchaTea2byAromaFingerprintingCombinedwithMultivariateChemometricAnalysisCHAOJin1,2,ZHOULingxin2,YINFeiyan2,LUOQian2,ZHAOMengmeng2,YUANYong2,WUHaoren2,LIPao1,JIANGLiwen1,*(1.CollegeofFoodScienceandTechnology,HunanAgriculturalUniversity,Changsha410128,China;2.NationalR&DCenterforTeaProcessing,HunanResearchCenterofTeaPlantationandProcessingTechnology,HunanTeaGroupCo.Ltd.,Changsha410126,China)Abstract:ThearomacomponentsoftwogradesofHuangjinchatea2wereinvestigatedbyusingheadspacesolid-phasemicroextraction(HS-SPME)coupledwithgaschromatography-massspectrometry(GC-MS)toestablishtheiraromafingerprints.Theresultswereanalyzedusingchemometrics.Totally81aromacomponentswereidentified,41ofwhichwerecommontoboth.Basedonthesecommonaromacomponents,thetwogradescouldbediscriminatedbyorthogonalpartialleastsquares-discriminationanalysis(OPLS-DA),andninedifferentialaromacomponentsbetweenthemwereidentifiedincludingheptanal,limonene,trans-β-ocimene,1-octanol,(E)-4,8-dimeth...