基金项目:浙江省医药卫生科技面上项目(2020KY326,2021KY359);浙江省基础公益研究计划项目(LGF19H200004)作者单位:312000绍兴市人民医院医学检验科(张丽红、胡良峰、王宏斌),病理科(杨烨)通信作者:张丽红,电子信箱:whb0575@163.comPYCR1在肝癌中的表达特点及其临床价值张丽红胡良峰杨烨王宏斌摘要目的探讨PYCR1在肝癌中的表达特点及其临床价值。方法运用生物信息学数据库分析PYCR1在肝癌组织中的表达及其与患者预后的关系;分别采用免疫组化和ELISA实验检测肝癌组织及患者外周血中PYCR1表达水平;应用ROC曲线评价PYCR1对肝癌的诊断价值。结果数据库分析发现,PYCR1在肝癌组织中的表达明显高于正常肝组织,PYCR1高表达肝癌患者预后较低表达患者差。肝癌组织(25/41,61.0%)PYCR1表达明显高于正常肝组织(1/25,4.0%),且其表达水平与患者年龄、性别无关。肝癌患者血清PYCR1蛋白水平明显高于肝良性疾病患者,且其表达水平与患者年龄、性别无关。PYCR1诊断肝癌的cut-off值为102.1pg/ml,曲线下面积(AUC)为0.711,敏感度为79.3%,特异性为64.7%。结论肝癌组织及患者外周血PYCR1高表达,其升高水平对肝癌具有一定的诊断及预后价值,可以作为潜在的肝癌标志物。关键词PYCR1肝癌受试者工作曲线临床价值中图分类号R44文献标识码ADOI10.11969/j.issn.1673-548X.2023.02.015ExpressionCharacteristicsandClinicalValueofPYCR1inHepatocellularCarcinoma.ZHANGLihong,HULiangfeng,YANGYe,etal.ShaoxingPeople′sHospital,Zhejiang312000,ChinaAbstractObjectiveToinvestigatetheexpressioncharacteristicsandclinicalvalueofPYCR1inhepatocellularcarcinoma(HCC).MethodsBioinformaticsdatabasewasusedtoanalyzetheexpressionofPYCR1inHCCanditsrelationshipwithprognosisofpatients.TheexpressionlevelsofPYCR1intissuesandperipheralbloodofpatientswithHCCweredetectedbyimmunohistochemistryandenzymelinkedimmunosorbentassay(ELISA),respectively.ThediagnosticvalueofPYCR1wasevaluatedbyreceiveroperatorcharacter-istic(ROC)curveinHCC.ResultsDatabaseanalysisshowedthattheexpressionofPYCR1inHCCtissueswassignificantlyhigherthanthatinnormallivertissues,andtheprognosisofHCCpatientswithhighexpressionofPYCR1wasworsethanthatofpatientswithlowexpressionofPYCR1.TheexpressionofPYCR1inHCCtissues(25/41,61.0%)wassignificantlyhigherthanthatinnormalliver...
