安徽农学通报2023年03期基因分子·生理生化·遗传育种·组织培养NaCl胁迫对白籽南瓜RBCs生理特性的影响孙梦影乔永旭*张永平杨丹李阮玲张周张莹莹（宿迁学院建筑工程学院/大运河文化带建设研究院宿迁分院生态文明研究中心，江苏宿迁223800）摘要为了探究根系边缘细胞（rootbordercells，RBCs）在植物抗盐胁迫中的作用和机制，以白籽南瓜种子为试材，研究0、50、100、150、200和250mmol/LNaCl对植株根长、RBCs数目、RBCs活率和RBCs黏胶层厚度的影响。结果表明，NaCl胁迫降低了植株根系的长度，随着NaCl浓度的增加，根系生长受到的抑制更明显；随着NaCl胁迫时间和浓度的增加，根系RBCs数目和活率也呈现不同程度的降低，其中200mmol/LNaCl胁迫36h的抑制效果最明显；但RBCs黏胶层厚度随NaCl胁迫浓度的增加而增加，200mmol/LNaCl胁迫的RBCs黏胶层厚度最大。总之，NaCl胁迫对白籽南瓜根系的生长有一定的伤害作用，RBCs通过自身死亡和增加黏胶层厚度来减轻这种伤害。关键词NaCl胁迫；白籽南瓜；根系边缘细胞；黏胶层中图分类号S642.9文献标识码A文章编号1007-7731（2023）03-0018-04EffectsofNaClonRootBorderCellsGenesisofPumpkinSeedlingsSUNMengyingQIAOYongxu*ZHANGYongpingYANGDanLIRuanlingZHANGZhouZHANGYingying（CollegeofConstructionEngineering,SuqianCollege/Beijing-HangzhouGrandCanalEcologicalCivilizationResearchCenter,SuqianJiangsu223800）AbstractTheeffectsof0、50、100、150、200and250mmol/LNaClonplantrootlength,RootBorderCells（RBCs）number,RBCsviabilityandRBCsmucilagelayerthicknesstakingthepumpkinseedsasmaterialwereinvestigatedinordertoexploretheroleandmechanismofRBCsinplantresistancetosaltstress.TheresultsshowedthatNaClstressreducedthelengthoftherootsystem,andtherootgrowthwasinhibitedmoreobviouslywiththeincreasingofNaClconcentration.WiththeincreasingofNaClstresstimeandconcentration,thenumberandviabilityofrootRBCsshowedadownwardtrend,especially200mmol/LNaClstressfor36hhadthemostobviousinhibitoryeffect.However,thethicknessofthemucilagelayerofRBCsincreasedwiththeincreaseofNaClstressconcentration,andthethicknessofthemucilagelayerofRBCsunder200mmol/LNaClstresswasthelargest.Inconclusion,NaClstresshadacertainharmfuleffectontherootgrowthofthepumpkin,andRBCsal...
