【山茱萸多糖炮制前后的化学研究】山茱萸的炮制方法是摘要：目的：比照炮制前后山茱萸多糖分子量和组成的变化，探讨炮制对其结构和组成的影响，阐述山茱萸多糖炮制增效的机理。方法：经脱脂、沸水提取，乙醇沉淀、Savage法脱蛋白得到粗多糖，再经DEAE-52阴离子交换树脂和SephadexG-200葡聚糖凝胶柱色谱别离得到纯化的山茱萸精多糖〔SZYS和SZYP〕，用红外光谱和紫外光谱分析其结构，凝胶色谱测定其相对分子量，HPLC测定其单糖组成。结果：别离得到了山茱萸多糖SZYS，其分子量为3.59×104，单糖组成为Glu∶Ara∶Rha=1∶1.51∶1.17；山茱萸多糖SZYP，分子量为1.16×104，单糖组成为Glu∶Ara∶Rha=1∶8.05∶1.78。结论：炮制前后山茱萸多糖分子量和单糖组成均发生变化，为山茱萸炮制增效的机理研究提供一定的依据。关键词：山茱萸；炮制；多糖；化学结构中图分类号：�R283.1文献标识码：A文章编号：1673-7717(2023)05-0950-03�ChemicalStudiesonPolysaccharideinCrudeandProcessedFructusCorni�DAIYanpeng1��，2，DINGXia1�，3，FUZiqin1��，2，CAIBaochang1��，2�(1.NanjingUniversityofChineseMedicine，Nanjing210029，Jiangsu,China;2.JiangsuKeyLaboratorryofChinese�MedicineProcessing,Najing210028,Jiangsu,China；3.NanjingAgricultureUniversity，Nanjing210095,Jiangsu,China)Abstract:Objective：ComparethechangesofthemolecularweightandcompositionofthepolysaccharideinFructusCornibeforeandafterprocessing.Exploretheeffectoftheprocessingtostructureandcompositionofthepolysaccharide.DescribethemechanismofpolysaccharidewhichisbettereffectiveinprocessedFructusCorni.Methods:Degreasing,hotwaterextraction,ethanolprecipitationandcolumnchromatographyonDEAE-celluloseandSephadexG-200wereusedtoisolateandpurifythepolysaccharidesinFructusCorni.IRandUVwereusedtoanalysisthestructureofthetwopurifiedpolysaccharide(SZYSandSZYP).HPGPCandHPLCwereusedtomeasurethemeanmolecularweightandmonosaccharidecomponentsofthepolysaccharides.Results:ThemolecularweightofpolysaccharideSZYSis3.59×104andthemonosaccharidecomponentsisGlu∶Ara∶Rha=1∶1.51∶1.17.ThemolecularweightofpolysaccharideSZYPis1.16×104andthemonosaccharidecompositionisGlu∶Ara∶Rha=1∶8.05∶1.78.Conclusion:Afterpro...
