Designation:D3457–87(Reapproved1991)e1StandardTestMethodforPreparationofMethylEstersFromFattyAcidsforDeterminationofFattyAcidCompositionbyGas-LiquidChromatography1ThisstandardisissuedunderthefixeddesignationD3457;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(e)indicatesaneditorialchangesincethelastrevisionorreapproval.e1NOTE—KeywordswereaddededitoriallyinAugust1991.1.Scope1.1Thistestmethodcoversaprocedureforconversionofanimalandvegetablefattyacidsintomethylestersofthefattyacidssuitableforanalysisbygas-liquidchromatography.1.2Thistestmethodisapplicabletoanimalandvegetablefattyacidshaving8to24carbonatoms.1.3Thisstandarddoesnotpurporttoaddressallofthesafetyproblems,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafetyandhealthpracticesanddeterminetheapplica-bilityofregulatorylimitationspriortouse.Forspecifichazardstatements,see6.4andNote1.2.ReferencedDocuments2.1ASTMStandards:D1193SpecificationforReagentWater2D1983TestMethodforFattyAcidCompositionbyGas-LiquidChromatographyofMethylEsters33.SummaryofTestMethod3.1Thistestmethodisbaseduponesterificationoffattyacidswithmethanol,usingborontrifluorideascatalyst.Themethylestersareextractedwithethyletherandrecoveredbyevaporationoftheether.3.2Aninternalstandardmaybeaddedifcertainconstitu-entsareconsideredlikelytobepresent(seeSection7).4.SignificanceandUse4.1ThistestmethodprovidesameansbywhichanimalorvegetablefattyacidsareconvertedintotheircorrespondingmethylesterssothatthefattyacidscanthenbeanalyzedbytheuseofTestMethodD1983.5.Apparatus5.1SeparatoryFunnels,125and60-mLwithTFE-fluorocarbonstopcocks.5.2PowderFunnel,widestem.5.3ErlenmeyerFlasks,2-Land25-mLcapacity.5.4Glass-StopperedStorageBottlesforBF3-methanol,500-mLcapacityorasrequired.5.5PlatformBalance,5-kgcapacity;smallestscaledivi-sionsrequiredare0.50g.5.6AnalyticalBalance,accurat...
