Designation:D5788−95(Reapproved2011)StandardGuideforSpikingOrganicsintoAqueousSamples1ThisstandardisissuedunderthefixeddesignationD5788;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thisguidecoversthegeneraltechniqueof“spiking”aqueoussampleswithorganicanalytesorcomponents.Itisintendedtobeapplicabletoabroadrangeoforganicmaterialsinaqueousmedia.Althoughthespecificdetailsandhandlingproceduresrequiredforalltypesofcompoundsarenotdescribed,thisgeneralapproachisgiventoserveasaguidelinetotheanalystinaccuratelypreparingspikedsamplesforsubsequentanalysisorcomparison.Guidanceisalsoprovidedtoaidtheanalystincalculatingrecoveriesandinterpretingresults.Itistheresponsibilityoftheanalysttodeterminewhetherthemethodsandmaterialscitedherearecompatiblewiththeanalytesofinterest.1.2Theproceduresinthisguidearefocusedon“matrixspike”preparation,analysis,results,andinterpretation.Theapplicabilityoftheseprocedurestothepreparationofcalibra-tionstandards,calibrationcheckstandards,laboratorycontrolstandards,referencematerials,andotherqualitycontrolmate-rialsbyspikingisincidental.Asample(thematrix)isfortified(spiked)withtheanalyteofinterestforavarietyofanalyticalandqualitycontrolpurposes.Whilethespikingofmultiplesampletestportionsisdiscussed,themethodofstandardadditionsisnotcovered.1.3Thisguideisintendedforuseinconjunctionwiththeindividualanalyticaltestmethodthatprovidesproceduresforanalysisoftheanalyteorcomponentofinterest.Thetestmethodisusedtodetermineananalyteorcomponent’sbackgroundleveland,againafterspiking,itsnowelevatedlevel.Eachtestmethodtypicallyprovidesproceduresnotonlyforsamples,butalsoforcalibrationstandardsoranalyticalcontrolsolutions,orboth.Theseproceduresincludeprepara-tion,handling,storage,preservation,andanalysistechniques.Theseproceduresareapplicablebyext...
