Designation:D8375−22StandardTestMethodforDeterminationofCannabinoidConcentrationinDriedCannabisandHempRawMaterialsusingLiquidChromatographyTandemMassSpectrometry(LC-MS/MS)1ThisstandardisissuedunderthefixeddesignationD8375;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thistestmethodallowsfortheconcentrationdetermi-nationofthecannabinoidslistedinTable1,andshallapplytoanydriedrawmaterialfromacannabisplant(Note1,Note2)regardlessofthetypeofcannabisplantfromwhichitwasderived.2Forthesakeofbrevity,theterm“cannabis”shallbeusedfromnowontorefertoanytypeofcannabisplantincludingthosethatcanbeclassifiedashemp.Theprocedureincludessub-samplingaground,homogeneoussample,extrac-tionwithmethanol:water(80:20,v:v),3,4dilutioninmethanolandanalysisbyliquidchromatographytandemmassspectrom-etry(LC-MS/MS).Themethodallowsforawide-rangeofsampleconcentrationstobedeterminedbyusinga1000-foldcalibrationrangeandtheoptiontoperformmultiplelevelsofsampledilution.Thecalibrationcurveispreparedinmethanoloverarangeof10ng⁄mLto10000ng/mLforallseventeencannabinoids,orasubsetofcannabinoidsifdesired,whilethesampleextractsaredilutedinmethanolintothecalibrationrange.3,4,5Forexample,a1/500dilutionofsampleextractsallowsconcentrationdeterminationoverarangeof0.5mg⁄gto500mg⁄gincannabis.Themethodwasvalidatedwithqualitycontrolsamplespreparedinmethanol,acandidatecertifiedreferencematerial(CRM),andrepeatanalysisofcannabinoidsamples.3NOTE1—Forthistestmethod,driedrawmaterialfromacannabisplantincludesoneormoreofinflorescence,leaves,orstems.NOTE2—Certainjurisdictionsorregulationsmayrequirespecificpartsoftheplanttobeincludedorexcludedforanalysisandthoseregulationswilltakeprecedencefortheselectionofplantparts.1.2Units—ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincl...
