ASTM_E_2406_-_09.pdf

Designation:E240609Standard Test Method forEvaluation of Laundry Sanitizers and Disinfectants for Usein High Efficiency Washing Operations1This standard is issued under the fixed designation E2406;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method is designed to evaluate sanitizing/disinfectant laundry detergents/additives for use in high effi-ciency(HE)automatic clothes washing operations that typi-cally utilize very low wash water volumes.This test method isdesigned to provide testing with representative vegetativebacteria but can also be designed to accommodate the testing offungi and viruses.NOTE1Test Method E2274 is the recommended method to evaluatesanitizing/disinfectant laundry detergent/additives for use in traditionalhigh wash water volume automatic clothes washing operations.1.2 Knowledge of microbiological techniques is requiredfor these procedures.1.3 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.NOTE2In this method,metric units are used for all applications,except for distance in which case inches are used.1.4 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:2D1193 Specification for Reagent WaterE177 Practice for Use of the Terms Precision and Bias inASTM Test MethodsE691 Practice for Conducting an Interlaboratory Study toDetermine the Precision of a Test MethodE1054 Test Methods for Evaluation of Inactivators of Anti-microbial AgentsE2274 Test Method for Evaluation of Laundry Sanitizersand Disinfectants2.2 AATCC Standard:AATCC 70-1997 Water Repellency;Tumble Jar DynamicAbsorption Test32.3 EPA Standard:DIS/TSS13 LaundryAdditivesDisinfectionandSanitization,U.S.Environmental Protection Agency,Of-fice of Pesticide Programs,April 198043.Terminology3.1 Definitions:3.1.1 active antimicrobial ingredienta substance added toa formulation intended specifically for the inhibition or inac-tivation of microorganisms.3.1.2 antimicrobial agent(s)an active ingredient designedto suppress the growth or action of microorganisms.3.1.3 carrier count controlprocedure used to determinethe initial number of microorganisms on a fabric carrierfollowing the inoculation and drying procedure.3.1.4 diluentsterile deionized water,sterile distilled wateror sterile synthetic AOAC hard water that may be used toprepare the active test formulation,vehicle control or productcontrol for use in the test procedure.3.1.5 diluted product solutiontest formulation,vehiclecontrol,or product control diluted to use concentration.3.1.6 neutralizationa process that results in quenching theantimicrobial activity of a test formulation.This may beachieved by dilution of the test formulation(s)to reduce theconcentration of the antimicrobials,or through the use ofchemical agents,called neutralizers,to suppress antibacterialactivity.3.1.7 numberscontrolinassessingsanitizerlevelperformance,procedure used to determine the number ofmicroorganisms remaining on the fabric carriers and in the1This test method is under the jurisdiction of ASTM Committee E35 onPesticides,Antimicrobials,and Alternative Control Agents and is the directresponsibility of Subcommittee E35.15 on Antimicrobial Agents.Current edition approved Nov.1,2009.Published December 2009.Originallyapproved in 2004.Last previous edition approved in 2004 as E2406 04.DOI:10.1520/E2406-09.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3Available from American Association of Textile Chemists and Colorists(AATCC),P.O.Box 12215,Research Triangle Park,NC 27709,http:/www.aatcc.org.4Available from United States Environmental Protection Agency(EPA),ArielRios Bldg.,1200 Pennsylvania Ave.,NW,Washington,DC 20460,http:/www.epa.gov.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 wash water following the test procedure in the presence of thediluent.This may also be performed using diluent or phosphatebuffer dilution water with surfactant.3.1.8 product controla formulation with or without anactive ingredient(s)used for comparison to the test formula-tion.3.1.9 test formulationa formulation containing an antimi-crobial agent(s).3.1.10 vehicle controlthe test formulation without theactive ingredient(s)used for comparison to the test formula-tion.3.1.11 wash waterth