Designation:E1241−05(Reapproved2013)StandardGuideforConductingEarlyLife-StageToxicityTestswithFishes1ThisstandardisissuedunderthefixeddesignationE1241;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thisguidedescribesproceduresforobtaininglaboratorydataconcerningtheadverseeffectsofatestmaterialaddedtodilutionwater—butnottofood—oncertainspeciesoffresh-waterandsaltwaterfishesduring28to120-day(dependingonspecies)continuousexposure,beginningbeforehatchandendingafterhatch,usingtheflow-throughtechnique.Thisguidewillprobablybeusefulforconductingearlylife-stagetoxicitytestswithsomeotherspeciesoffish,althoughmodi-ficationsmightbenecessary.1.2Othermodificationsoftheseproceduresmightbejusti-fiedbyspecialneedsorcircumstances.Althoughusingappro-priateproceduresismoreimportantthanfollowingprescribedprocedures,resultsoftestsconductedusingunusualproceduresarenotlikelytobecomparabletoresultsofmanyothertests.Comparisonofresultsobtainedusingmodifiedandunmodifiedversionsoftheseproceduresmightprovideusefulinformationconcerningnewconceptsandproceduresforconductingearlylife-stagetoxicitytestswithfishes.1.3Theseproceduresareapplicabletoallchemicals,eitherindividuallyorinformulations,commercialproducts,orknownmixtures,thatcanbemeasuredaccuratelyatthenecessaryconcentrationsinwater.Withappropriatemodifica-tionstheseprocedurescanbeusedtoconducttestsontemperature,dissolvedoxygen,andpHandonsuchmaterialsasaqueouseffluents(seeGuideE1192),leachates,oils,par-ticulatematter,sediments,andsurfacewaters.1.4Thisguideisarrangedasfollows:SectionReferencedDocuments2Terminology3SummaryofStandard4SignificanceandUse5.1Hazards6Apparatus7Facilities7.1ConstructionMaterials7.2MeteringSystem7.3TestChambersandIncubationCups7.4Cleaning7.5Acceptability7.6DilutionWater8Requirements8.1Source8.2Treat...
