Designation:E1711−12StandardGuideforMeasurementofBehaviorDuringFishToxicityTests1ThisstandardisissuedunderthefixeddesignationE1711;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thisguidecoverssomegeneralinformationonmethodsforqualitativeandquantitativeassessmentofthebehavioralresponsesoffishduringstandardlaboratorytoxicityteststomeasurethesublethaleffectsofexposuretochemicalsub-stances.Thisguideismeanttobeanadjuncttotoxicitytestsandshouldnotinterferewiththosetestprocedures.1.2Behavioraltoxicosisoccurswhenchemicalorotherstressfulconditions,suchaschangesinwaterqualityortemperature,induceabehavioralchangethatexceedsthenormalrangeofvariability(1).Behaviorincludesalloftheobservable,recordable,ormeasurableactivitiesofalivingorganismandreflectsgenetic,neurobiological,physiological,andenvironmentaldeterminants(2).1.3Behavioralmethodscanbeusedinbiomonitoring,inthedeterminationofno-observed-effectandlowest-observed-effectconcentrations,andinthepredictionofhazardouschemicalimpactsonnaturalpopulations(3).1.4Thebehavioralmethodsdescribedinthisguideincludelocomotoryactivity,feeding,andsocialresponses,whicharecriticaltothesurvivaloffish(4).1.5Thisguideisarrangedasfollows:SectionNumberScope1ReferencedDocuments2Terminology3SummaryofGuide4SignificanceandUse5Interferences6SafetyPrecautions7ResponsesMeasured8TestOrganisms9Facility10QualitativeBehavioralAssessmentMethod11QuantitativeBehavioralMeasurements12ExperimentalDesign13CalculationofTestResults14Report151.6Thevaluesstatedininch-poundunitsaretoberegardedasstandard.ThevaluesgiveninparenthesesaremathematicalconversionstoSIunitsthatareprovidedforinformationonlyandarenotconsideredstandard.1.7Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstan...