Designation:E1367−03(Reapproved2014)StandardTestMethodforMeasuringtheToxicityofSediment-AssociatedContaminantswithEstuarineandMarineInvertebrates1ThisstandardisissuedunderthefixeddesignationE1367;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope*1.1Thistestmethodcoversproceduresfortestingestuarineormarineorganismsinthelaboratorytoevaluatethetoxicityofcontaminantsassociatedwithwholesediments.Sedimentsmaybecollectedfromthefieldorspikedwithcompoundsinthelaboratory.GeneralguidanceispresentedinSections1–15forconductingsedimenttoxicitytestswithestuarineormarineamphipods.Specificguidanceforconducting10-dsedimenttoxicitytestswithestuarineormarineamphipodsisoutlinedinAnnexA1andspecificguidanceforconducting28-dsedimenttoxicitytestswithLeptocheirusplumulosusisoutlinedinAnnexA2.1.2Proceduresaredescribedfortestingestuarineormarineamphipodcrustaceansin10-dlaboratoryexposurestoevaluatethetoxicityofcontaminantsassociatedwithwholesediments(AnnexA1;USEPA1994a(1)).Sedimentsmaybecollectedfromthefieldorspikedwithcompoundsinthelaboratory.Atoxicitymethodisoutlinedforfourspeciesofestuarineormarinesediment-burrowingamphipodsfoundwithinUnitedStatescoastalwaters.ThespeciesareAmpeliscaabdita,amarinespeciesthatinhabitsmarineandmesohalineportionsoftheAtlanticcoast,theGulfofMexico,andSanFranciscoBay;Eohaustoriusestuarius,aPacificcoastestuarinespecies;Leptocheirusplumulosus,anAtlanticcoastestuarinespecies;andRhepoxyniusabronius,aPacificcoastmarinespecies.Generally,themethoddescribedmaybeappliedtoallfourspecies,althoughacclimationproceduresandsometestcondi-tions(thatis,temperatureandsalinity)willbespecies-specific(Sections12andAnnexA1).Thetoxicitytestisconductedin1-Lglasschamberscontaining175mLofsedimentand775mLofoverlyingseawater.Exposureisstatic(thatis,waterisnotrenewed),andtheani...
