Designation:D932−15StandardPracticeforFilamentousIronBacteriainWaterandWater-FormedDeposits1ThisstandardisissuedunderthefixeddesignationD932;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thispracticecoversthedeterminationoffilamentousironbacteria(FIB)byexaminationunderthemicroscope.Thepracticeprovidesfortheidentificationofthefollowinggeneraofbacteriafoundinwaterandwater-formeddeposits:Siderocapsa,Gallionella(Dioymohelix),Sphaerotilus,Crenothrix,Leptothrix,andClonothrix.1.2ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.3Thisstandarddoesnotpurporttoaddressthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.2.ReferencedDocuments2.1ASTMStandards:2D887PracticesforSamplingWater-FormedDepositsD1129TerminologyRelatingtoWaterD1193SpecificationforReagentWaterD3370PracticesforSamplingWaterfromClosedConduitsD5465PracticeforDeterminingMicrobialColonyCountsfromWatersAnalyzedbyPlatingMethods3.Terminology3.1Definitions—Fordefinitionsoftermsusedinthispractice,refertoTerminologyD1129.4.SummaryofTestMethod4.1Theironbacteriaaregenerallyfilamentous,typicallyfoundinfreshwater,andfrequentlysurroundedbyasheathwhichisusuallyencrustedwithironormanganese,orboth(1,2).3However,Starkey(3)reportsanothertypewhichisclassifiedamongthetruebacteria.Detectionandidentificationisaccomplishedbymicroscopicexaminationofsedimentfromthesample.4.2Thispracticeprovidesaqualitativeindicationofthedensityofthefilamentousironbacteriaandtheseverityofthecloggingprobleminpipescausedbythesebacteria.5.SignificanceandUse5.1Filamentousironbacteriaisageneralclassificationformicroorganismsthatutilizeferrou...
