兔脂肪干细胞体外成骨诱导培养及成骨活性鉴定的实验研究张开刚1张月东1王玫2（1.泰安市中心医院骨科，山东泰安2710002.泰山医学院，山东泰安271016）【摘要】目的体外分离培养兔脂肪干细胞，在成骨诱导条件下，鉴定其成骨活性。方法取4月龄新西兰大白兔腹股沟区脂肪,Ⅰ型胶原酶消化,分离、培养及传代原细胞,将第3代ADSCs消化后，加入成骨培养液中诱导分化,分别行碱性磷酸酶染色、茜素红染色、VonKossa（钙结节）染色鉴定分化结果.结果体外分离培养的细胞增殖活跃，成骨诱导后检测碱性磷酸酶染色、茜素红染色、VonKossa（钙结节）染色均呈阳性表达。结论脂肪干细胞具有成骨分化能力,是一种理想的骨组织工程种子细胞。【关键词】脂肪干细胞；成骨诱导；种子细胞Anexperimentalstudyofosteogeniccultureinvitroandidentificatingtheosteogenicactivityofrabbitadipose-derivedstemcellsZHANGKaigang1,ZHANGYuedong1,WangMei2（1.DepartmentofOrthopedicSurgery,TaianCenterHospital,ShandongTaian,2710002.TaishanMedicalCollege，ShandongTaian271016）【Abstract】ObjectiveSeparatingandCulturingrabbitadipose-derivedstemcells，identificatingtheosteogenicactivityinosteogenicconditions.MethodsExtractinginguinalfatofNewZealandwhiterabbitsaged4months,UsingIcollagenasedigestiong,separating,culturingandpassagingoftheoriginalcells,addingosteogenicmediumtoinduceosteogenicdifferentiationafterdigestingthe3rdgenerationADSCs,thenidentificatingthedifferentiationresultsbyalkalinephosphatasestaining、alizarinredstaining、VonKossa(calciumnodules)staining.ResultsThecellsofSeparatingandCulturinginvitrowereactive,thealkalinephosphatasestaining、alizarinredstainingandVonKossa(calciumnodules)stainingwerebothpositiveexpressionafterosteogenic.ConclusionAdiposederivedstemcellshavetheabilityofOsteogenicdifferentiation,theyareanidealseedcellsforbonetissueengineering.【Keywords】Adipose-DerivedStemCells;Osteogenic;SeedCellsAnExperimentalstudyofAdiposestemcellsculturedinvitroosteogenicandosteogenicactivityidentificationZHANGKaigang1,ZHANGYuedong1,WangMei2（1.DepartmentofOrthopedicSurgery,TaianCenterHospital,ShandongTaian,2710002.TaishanMedicalCollege，ShandongTaian271016）...
