农业环境科学学报JournalofAgro⁃EnvironmentScience2022，41（12）:2688-26932022年12月张丽芳，许旖旎，曹阳，等.哈茨木霉菌HNA12在防控玉米黄曲霉毒素污染中的应用[J].农业环境科学学报,2022,41（12）：2688-2693.ZHANGLF,XUYN,CAOY,etal.TrichodermaharzianumHNA12anditsapplicationincontrollingaflatoxincontaminationinmaize[J].JournalofAgro-EnvironmentScience,2022,41（12）：2688-2693.开放科学OSID哈茨木霉菌HNA12在防控玉米黄曲霉毒素污染中的应用张丽芳1，许旖旎1，曹阳1，刘力强2（1.江苏食品药品职业技术学院，江苏淮安223003；2.河北科技大学，石家庄050018）TrichodermaharzianumHNA12anditsapplicationincontrollingaflatoxincontaminationinmaizeZHANGLifang1,XUYini1,CAOYang1,LIULiqiang2（1.JiangsuFoodandPharmaceuticalScienceCollege,Huai′an223003,China;2.HebeiUniversityofScienceandTechnology,Shijiazhuang050018,China）Abstract：Tocontrolmycotoxincontaminationfromthesource,highthroughputscreeningandbiologicalcontrolofantagonisticmicroorganismsagainstaflatoxincontaminationofmaizewerecarriedoutinthisstudy.Intotal,1055strainsofTrichodermawereisolatedfromhealthymaizeseedscollectedfromJiangsu,Shandong,ShanxiandHenanprovinces.Amongthem,10isolatesshowedantagonisticactivitywithabandwidthofmorethan8mm,andtheinhibitionratewasrangedfrom36.5%to90.0%.Vivoexperimentsshowedthat7TrichodermastrainshadpotentinhibitoryeffectsagainstA.flavuswitharateof44.2%to87.0%,andtheinhibitionrateagainstaflatoxinrangedfrom40.5%to85.0%.Amongthestrainstested,thecontrolefficiencyofHNA12,T40-3,andT85-5againstA.flavuswas87.0%,68.8%,and64.5%,respectively.Thestrainswiththehighestinhibitionratesof85.0%,75.2%,and68.5%againstaflatoxinwereHNA12,T21-1,andT40-3,respectively.FieldtrialresultsshowedthatHNA12hadthehighestgrowthpromotionandaflatoxininhibitionratesof10.5%and90.5%,respectively,indicatingagreatpotentialforbiocontrol.Basedonmorphologicalandmolecularbiologicalanalyses,strainHNA12wasidentifiedasTrichodermaharzianum.AntagonisticmechanismtestshowedthatthefermentationproductandtheproteinextractofthestrainHNA12hadastronginhibitionabilityon...
