第46卷第1期2023年1月河北农业大学学报JOURNALOFHEBEIAGRICULTURALUNIVERSITYVol.46No.1Jan.2023核桃JrOFP基因家族鉴定及其响应冷胁迫基因的转录表达分析刘凯1,李洋1,纪莲莲1,林娜1,王红霞2,王梦娜1,王娇1,孔祥文1(1.滨州职业学院,山东滨州,256603;2.河北农业大学园艺学院,河北保定071001)摘要:OFP(OVATEFamilyProtein)在植物调控响应逆境过程有重要作用。本研究基于核桃全基因组数据对其OPF基因家族成员进行筛选鉴定,并对其成员结构进行生物信息分析,同时对该家族成员进行时空转录水平分析。结果表明,核桃OFP基因家族包含21个成员,编码21条蛋白,均为亲水性蛋白;均含有典型的DUF623结构域;系统进化发现,该基因家族成员分为4个亚组。冷胁迫后经转录组分析,21个成员对冷胁迫均具有不同程度的响应,并在不同组织中存在特异性表达。本研究为进一步解析核桃OPF基因家族成员在响应冷胁迫过程中的分子机制提供了理论支撑。关键词:核桃;冷胁迫;OPF基因家族;转录分析中图分类号:S664.1开放科学(资源服务)标识码(OSID):文献标志码:AIdentificationandtranscriptionalanalysisofJrOFPgenefamilyinresponsetochillingstressinwalnutLIUKai1,LIYang1,JILianlian1,LINNa1,WANGHongxia2,WANGMengna1,WANGJiao1,KONGXiangwen1(1.BinZhouPolytechnic,Binzhou,256603,China;2.CollegeofHorticulture,HebeiAgriculturalUniversity,Baoding071001,China)Abstract:OFPplaysanimportantroleinplantregulationandresponsetostressinthisstudy,themembersoftheOFPgenefamilywerescreenedoutofthewholegenomeofwalnut,andthebioinformaticsanalysisofthestructureofthememberswascarriedout.Atthesametime,thetranscriptionlevelsofthefamilymemberswereanalyzedundercoldstressatdifferenttimesandindifferenttissues.TheresultsshowedthatthewalnutOFPgenefamilycontained21members,encoding21proteins,thatwereallhydrophilic.TheproteinsallcontainedatypicalDUF623domain.Themembersweredividedinto4subgroupsphylogenetically.Thetranscriptomedatashowedthat21membersrespondedtodifferentdegreesofcoldstressatdifferenttimes,andwereexpressedindifferenttissues.TheresultsofthisstudywillprovidetheoreticalsupportforfurtheranalysisofthemolecularmechanismofwalnutOPFgenefamilymembersinresponsetochillingstressandgrowth...