第53卷第2期2023年2月Vol.53No.2Feb.2023·157·日用化学工业（中英文）ChinaSurfactantDetergent&CosmeticsInchemicoskinsensitisation:AminoacidDerivativeReactivityAssay（ADRA）transformationandvalidationLiShen*,XuemeiWang,HuiwenZhang,YaqiongSuo,JingWu,ShuxiaXing（NationalInstitutesforFoodandDrugControl,Beijing100050,China）Abstract:AskinsensitizerreferstoasubstancethatwillleadtoanallergicresponsefollowingrepeatedskincontactasdefinedbytheUnitedNationsGloballyHarmonizedSystemofClassificationandLabellingofChemicals.ThecurrentknowledgeofthechemicalandbiologicalmechanismsassociatedwithskinsensitisationhasbeensummarisedasanAdverseOutcomePathway（AOP）startingwithamolecularinitiatingeventthroughintermediateeventstotheadverseeffect,namelyallergiccontactdermatitis.Usingcysteineandlysinederivativesinsteadofskintissue,simulatingthereactionofskinproteinswithcompounds,classifyingthesubstancesbycalculatingthepercentageofdepletionvalueafterthereactionofcysteineandlysinederivativestodistinguishskinsensitizersanddesensitizers,thismethodiscalledAminoacidDerivativeReactivityAssay（ADRA）.Inthisstudy,tenknownallergenswereselectedtoexploretheaccuracyofthesensitivityevaluationofADRAmethod,andverifiedaccuracybyDPRAthatseveralsubstanceswererandomlyselectedamongthetensubstances.Theresultsshowthatthesensitivitydeterminationresultsof10substancesareconsistentwiththesensitivityofsamples,andtheaccuracyofdeterminationofADRAsensitivityisgood.Keywords:skinsensitisation;alternativesexperiment;ADRAReceived:February14,2022;Revised:February3,2023.*Correspondingauthor.Tel.:+86-18720913402,E-mail:842585696@qq.com.DOI：10.3969/j.issn.1001-1803.2023.02.005·158·第53卷开发与应用日用化学工业（中英文）化妆品的使用特点为反复多次接触皮肤，因此皮肤过敏性评价是化妆品毒理学评价的重要内容。以往，化学物质潜在致敏及其效力数据需要从动物实验中获取，随着实验动物福利3R原则日趋受到重视，此法在现如今的环境下已经越来越不可取，因此合适的替代方法对于化妆品安全性评价尤为重要。人们对于皮肤致敏有关的化学和生物学机制的当前知识已概...
