农业环境科学学报JournalofAgro⁃EnvironmentScience2022，41（12）:2772-27782022年12月鲍莹，严梦圆，吴萌，等.腐殖酸配施壳聚糖调控设施番茄土壤细菌群落[J].农业环境科学学报,2022,41（12）：2772-2778.BAOY,YANMY,WUM,etal.Humicacidcombinedwithchitosanregulatesbacterialcommunitiesintomatofacilitysoil[J].JournalofAgro-EnvironmentScience,2022,41（12）：2772-2778.开放科学OSID腐殖酸配施壳聚糖调控设施番茄土壤细菌群落鲍莹1，2，严梦圆1，2，吴萌1，2，李桂龙1，2，刘凯1，2，李忠佩1，2*（1.土壤与农业可持续发展国家重点实验室，中国科学院南京土壤研究所，南京210008；2.中国科学院大学，北京100049）HumicacidcombinedwithchitosanregulatesbacterialcommunitiesintomatofacilitysoilBAOYing1,2,YANMengyuan1,2,WUMeng1,2,LIGuilong1,2,LIUKai1,2,LIZhongpei1,2*（1.StateKeyLaboratoryofSoilandSustainableAgriculture,InstituteofSoilScience,ChineseAcademyofSciences,Nanjing210008,China;2.UniversityofChineseAcademyofSciences,Beijing100049,China）Abstract：Inordertoexploremoreefficientgreencontrolmethodsforbacterialwiltintomatofacilities,wecollectedinfectedsoil,addedbiologicalmodifiers,humicacidand/orchitosan,andincubateditat26℃for10weeks.Theresultsshowedthatthesoilbacterialcommunitystructurewassignificantlydifferentwiththeco-applicationofhumicacidandchitosan（HC）comparedwiththecontrolandsingleapplicationtreatments（P<0.05）.UnderHCtreatment,therelativeabundanceofthebacterialwiltpathogenRalstoniaspp.wassignificantlydecreased,whiletherelativeabundanceofbeneficialmicroorganisms,Streptomycesspp.,Kitasatosporaspp.,Catenulisporaspp.,andTerracidiphilusspp.,wassignificantlyincreased（P<0.05）.Theco-occurrencenetworkresultsshowedthatthenetworkconnectivity,clusteringcoefficient,andaveragedegreeofHCsubnetworksweresignificantlyimproved（P<0.05）andthereweredirectandindirectinhibitoryeffectsbetweenbeneficialmicroorganismsandRalstoniaspp.;theindirectinhibitoryeffectwasdominant.Ourresultsindicatedthattheco-applicationofhumicacidandchitosanenhancedtheinteractionbetweensoilbacterialcommunitiesandinhibitedthegrowthofRalstoniasp...
