·46·MedicalLaboratoryScienceandClinics,2024,Vol.35,No.1医学检验与临床2024年第35卷第1期doi:10.3969/j.issn.1673-5013.2024.01.011Rh系统抗-D与抗-E抗体共同致新生儿溶血病的研究分析吴晓春姜坤于晓梅孙福廷←（潍坊市人民医院输血科，山东潍坊261041)[摘要目的：研究1例Rh系统2种不规则抗体（抗-D、抗-E）同时存在导致新生儿溶血病。方法：通过临床资料提供，对产妇进行血型分析，不规则抗体筛查及抗体特异性鉴定，抗体效价分析；对新生儿进行直接抗人球蛋白试验、游离试验、放散试验及抗体特异性分析。结果：产妇不规则抗体阳性，抗体为Rh系统抗-D与抗-E，IgG抗-D效价为512，IgG抗-E效价为2；新生儿直接抗人球蛋白试验阳性、放散试验检出抗-D和抗-E抗体，游离试验检出抗-D抗体。结论：经新生儿三项实验诊断新生儿溶血病成立，积极给予对症治疗，患儿康复出院。[关键词]新生儿溶血病；RhD阴性；抗-D；抗-ETheresearchandanalysisofhemolyticdiseaseofnewborncausingbyanti-Dwithanti-EinRhsystemWUXiao-chun,JIANGKun,YUXiao-mei,etal(WeifangPeople'sHospital,ShandongWeifang261041)[Abstract]Objective:Toinvestigatedonecaseofhemolyticdiseaseofthenewborncausedbysimultaneousoftwoirregularantibodies(anti-Dandanti-E)inRhsystem.Methods:Providedbyclinicaldata,Afterbloodgroupidentificationandirregularantibodyscreeningofpregnantwoman,determiningtiterofantibodybymeansofimmunoserology;Directantiglobulintest,antibodyreleasetest,freeantibodytestandfurtherantibodyidentificationconductingforthenewborn.Results:Theirregularantibodyispositivecombininganti-Dwithanti-EinRhsystem,theIgGtiterofantibodywas512and2;Directantiglobulintestispositive,antibodyreleasetestappearinganti-Dandanti-E,anti-Dpresentedinfreeantibodytest.Conclusion:Thediagnosisofhemolyticdiseaseofnewbornwasestablishedandthechildrecoverydischargedfromhospitalsmoothlygivedbysymptomatictreatmentactively.[Keywords]Hemolyticdiseaseofnewborn;RhDnegative;Anti-D;Anti-E新生儿溶血病（Hemolyticdiseaseofnewborn,HDN）指母婴血型不合导致胎儿或新生儿发生免疫性溶血性疾病，红细胞间发生同种免疫是致病的主要原因"，胎儿红细胞经胎盘进人母体，刺激母体产生针对该抗原的同种抗体，该抗体又经胎盘进入胎儿体内参与血液循环...
