JournalofVisualizedExperimentswww.jove.comCopyright©2008JournalofVisualizedExperimentsPage1of2MaterialsListfor:SelectiveLabellingofCell-surfaceProteinsusingCyDyeDIGEFluorMinimalDyesAsaHagner-McWhirter1,MariaWinkvist1,StephanieBourin1,RitaMarouga11ResearchandDevelopment,GEHealthcareBio-SciencesABCorrespondenceto:AsaHagner-McWhirteratasa.hagner-mcwhirter@ge.comURL:http://www.jove.com/video/945DOI:doi:10.3791/945MaterialsNameTypeCompanyCatalogNumberCommentsCyDyeDIGEKit,2nmolReagentGEHealthcare28-9345-302-DQuantKitReagentGEHealthcare80-6484-51IPGBufferpH3-11NLReagentGEHealthcare17-6004-40ImmobilineDryStrippH3-11NL,24cmReagentGEHealthcare17-6003-77IPGboxToolGEHealthcare28-9334-65IPGboxkitToolGEHealthcare28-9334-92DeStreakRehydrationsolutionReagentGEHealthcare17-6003-19ImmobilineDryStripCoverFluidReagentGEHealthcare17-1335-01EttanIPGphor3IEFUnitInstrumentGEHealthcare11-0033-64EttanIPGphorManifoldInstrumentcomponentGEHealthcare80-6498-38EttanDALTtwelveGelCasterToolGEHealthcare80-6467-22EttanDALTtwelveSeparationUnitandPowerSupply/ControlUnitInstrumentGEHealthcare80-6466-46230Vunit:80-6466-27Typhoon9410VariableModeImagerInstrumentGEHealthcare63-0055-81EttanSpotPickerInstrumentGEHealthcare18-1145-28EttanDigesterInstrumentGEHealthcare18-1142-68EttanSpotterInstrumentGEHealthcare18-1142-67EttanMALDI-TOFInstrumentGEHealthcare18-1142-33DeCyder2-DDifferentialAnalysisSoftwareOtherGEHealthcare28-4012-01ImageQuantAnalysisSoftwareOtherGEHealthcare28-9236-62UreaReagentGEHealthcare17-1319-01CHAPSReagentGEHealthcare17-1314-01PlusOneDithiothreitol(DTT)ReagentGEHealthcare17-1318-01BromophenolBlueReagentGEHealthcare17-1329-01TrisReagentGEHealthcare17-1321-01SodiumDodecylsulfate(SDS)ReagentGEHealthcare17-1313-01PlusOneGlycerol87%ReagentGEHealthcare17-1325-01JournalofVisualizedExperimentswww.jove.comCopyright©2008JournalofVisualizedExperimentsPage2of2PlusOneReadySolIEF40%T,3%CReagentGEHealthcare17-1310-01PlusOneTEMEDReagentGEHealthcare17-1312-01PlusOneAmmoniumPersulfateReagentGEHealthcare17-1311-01PlusOneGlycineReagentGEHealthcare17-1323-012-DSamplePrepformembraneproteinsReagentPierce,ThermoScientific89864StreptomycinsulphateReagentInvitrogen15140-122
